| 摘要: |
| [目的]探索甘蓝型油菜雄性不育系B2子叶和下胚轴的最佳离体培养条件。[方法] 以甘蓝型油菜雄性不育系B2无菌苗的子叶和下胚轴为外植体,研究了苗龄、预培养时间、预培养基和分化培养基中激素配比等对甘蓝型油菜雄性不育系B2子叶和下胚轴高频再生的影响。[结果] 4因素对甘蓝型油菜雄性不育系B2子叶和下胚轴的再生均有明显影响。4 d苗龄,在MS+6-BA 1mg/L+2,4-D 1mg/L预培养基上预培养3 d后诱导分化,子叶在添加 NAA0。02 mg/L+6-BA 3。0 mg/L的分化培养基中分化率最高,达80。45%,下胚轴在添加NAA 0。05 mg/L+6-BA 3。0 mg/L的分化培养基中分化率最高,达69。33%。[结论] 建立了甘蓝型油菜雄性不育系B2子叶和下胚轴稳定高效的再生体系,为其遗传转化奠定了基础。 |
| 关键词: 甘蓝型油菜 雄性不育系 子叶 下胚轴 高频再生 |
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| 基金项目:陕西省科技攻关项目（2003K03-G1-04） |
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| Establishment of high-frequency shoot regeneration system of cotyledons and hypocotyls from male sterile line B2 of B.napus L. |
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| Abstract: |
| 【Objective】 The study explored the best in vitro culture conditions of cotyledons and hypocotyls from male sterile line B2 of Brassica napus L..【Method】 With the cotyledons and hypocotyls from male sterile line B2 of Brassica napus L.as explants,the effects of four factors, donor plant age,pre-culture time and hormone combination in pre-culture medium and that in differentiation medium,on high-frequency shoot regeneration of hypocotyls and cotyledons from line B2 were studied in the experiment.【Result】 All four factors had significant effects on the shoot regeneration of the cotyledons and hypocotyls from male sterile line B2.Inducing differentiation in differentiation medium at 4d plant age following 3d culture in pre-culture medium containing 6-BA 1 mg/L+2,4-D 1 mg/L,showed highest differentiation rates of cotyledons and hypocotyls were respectively 80.45% in medium containing NAA 0.02 mg/L+6-BA 3.0 mg/L and 69.33% in medium containing NAA 0.05 mg/L+6-BA 3.0 mg/L.【Conclusion】 Stable and efficient regeneration systems for cotyledons and hypocotyls from male sterile line B2 were established,which laid a foundation for genetic transformation of line B2. |
| Key words: Brassica napus L male sterile line cotyledon hypocotyl high-frequency regeneration |
