| 摘要: |
| [目的]研究不同水稻土来源的微生物群落,在利用甲酸盐、乙酸盐、丙酸盐、丙酮酸盐、柠檬酸盐及琥珀酸盐作为惟一碳源时对异化Fe(Ⅲ)还原的影响.[方法]采用四川、吉林和江西3种不同水稻土浸提液接种作为微生物群落来源,以人工合成的Fe(OH)3为惟一的电子受体,不同有机酸作为惟一碳源,在25℃厌氧条件下培养,定期测定Fe(Ⅱ)生产量并对测定结果用Logistic模型进行分析.[结果]四川水稻土中的微生物群落在Fe(OH)3还原过程中对所有供试碳源均表现出较高的利用活性,其对应的Fe(Ⅱ)累积量较高;江西水稻土中的微生物群落对碳源的响应很低,且几乎不能利用丙酸盐进行Fe(OH)3的还原;而吉林水稻土中的微生物群落在Fe(OH)3还原过程中几乎不能利用琥珀酸盐作为电子供体.[结论]3种微生物群落在Fe(OH)3还原过程中对供试碳源利用上的不同,反映了其微生物群落组成和代谢功能上的差异.3种微生物群落均能较快地利用丙酮酸盐还原Fe(OH)3,说明Fe(Ⅲ)还原微生物在碳源利用上具有共性. |
| 关键词: 异化Fe(Ⅲ)还原 有机酸 水稻土 微生物群落 |
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| 基金项目:国家自然科学基金项目(40271067，40741005)；西北农林科技大学创新团队项目 |
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| Iron reducing characteristics of organic acid utilization with microbes in different paddy soil |
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| Abstract: |
| 【Objective】 The effect of formate,acetate,propionate,pyruvate,citrate and succinate as sole carbon substrates on dissimilatory iron reduction in incubations with microbial communities from different paddy soils has been investigated. 【Method】 Paddy soil water extracts from Sichuan,Jilin and Jiangxi were sued as inoculums,ferrihydrite synthesized artificially as sole electronic accepter.Cultured under anaerobic condition at 25 ℃,yield of Fe(Ⅱ) was mensurated termly and analyzed by Logisitic model. 【Result】 Fe(Ⅲ) reduction was clearly observed in the utilizations of all carbon substrates tested by microbes from Sichuan paddy soil,contrarily,microbes from Jiangxi paddy soil had lower response,especially could hardly use propionate.Succinate could not be used by microbes from Jilin paddy soil. 【Conclusion】 Distinct Fe(Ⅲ) reductions in the utilization of all organic acid by different microbes reflect different compositions and metabolic function of the microbial communities from the three paddy soils.But the best utilizations of pyruvate indicates some common characteristics of dissimilatory Fe(Ⅲ) reducing bacteria in paddy soil. |
| Key words: dissimilatory Fe(Ⅲ) reduction organic acid paddy soil microbial community |
