| 摘要: |
| [目的]探讨屎肠球菌对瘤胃发酵的影响,以此评价屎肠球菌能否作为直接饲用微生物用于反刍动物的生产.[方法]利用持续动态人工瘤胃装置,采用完全随机试验设计,以不添加屎肠球菌发酵液为对照,研究了添加1.25×106和6.25×106mL-1屎肠球菌发酵液对瘤胃液pH、微生物蛋白(MCP)、氨态氮(NH3-N)、乙酸、丙酸、丁酸和总挥发性脂肪酸(TVFA)浓度的影响.[结果]添加屎肠球菌对瘤胃pH值和MCP无显著影响(P>0.05),但能显著提高瘤胃NH3-N浓度(P<0.01);与对照组相比,1.25×106mL-1屎肠球菌液添加组显著提高了瘤胃液的乙酸与总挥发性脂肪酸浓度(P<0.05),而添加屎肠球菌对瘤胃丙酸和丁酸浓度及乙酸与丙酸的比例没有显著影响(P>0.05).[结论]添加一定量的屎肠球菌,可在不改变瘤胃发酵类型的情况下促进饲料中碳水化合物的消化. |
| 关键词: 人工瘤胃 屎肠球菌 瘤胃发酵 |
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| 基金项目:国际科技合作重点项目“奶牛安全饲用微生物及其人工瘤胃评价技术研究”（2004DFA06700） |
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| Effect of artificial rumen evaluating Enterococcus faecium on ruminal fermentation |
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| Abstract: |
| 【Objective】 This experiment was done to study the effect of Enterococcus faecium on ruminal fermentation so as to evaluate whether Enterococcus faecium can be used as direct-fed microbe in ruminant according the result of this study.【Method】 The experiment used completely random trial design and the artificial rumen simulation instrument.The group which didn't supply Enterococcus faecium was control group and the effect of supply 1.25×106mL-1 and 6.25×106mL-1 Enterococcus faecium on rumen fluid pH value,microbial crude protein(MCP),NH3-N and the VFA concentration was analyzed.【Result】 Ruminal pH value and MCP were not significantly affected by supplementing Enterococcus faecium（P＞0.05）.However,supplementation with Enterococcus faecium could increase ruminal NH3-N concentration（P＜0.01）.Compared with control group,acetate and total VFA concentration in 1.25×106mL-1 Enterococcus faecium group were higher than that of the control group.However,the concentration of propionate and butyrate,and the ratio of acetate to propionate were not significantly affected by supplementation of Enterococcus faecium（P＞0.05）.【Conclusion】 The results implicated that Enterococcus faecium did not change the type of rumen fermentation,but appropriate dose of supplementation could improve rumen digestion of carbohydrate in feed. |
| Key words: artificial rumen Enterococcus faecium ruminal fermentation |
