| 摘要: |
| [目的]通过对奶牛MC1R基因核苷酸序列的分析和蛋白结构的预测,探讨奶牛红白花毛色形成的分子机制.[方法]采用PCR-RFLP技术对奶牛MC1R基因进行分型,利用生物信息学方法对其蛋白结构特征进行模拟分析.[结果]共检测出与毛色表型有关的3种基因型,其中EE基因型在黑白花奶牛中占优势,ee基凶型在红白花奶牛中占优势.蛋白结构预测结果显示,ee基因型个体的缺失突变使蛋白质翻译提前终止,进而引发二级结构改变,导致红白花奶牛个体的出现.[讨论]E等位基因主要与黑色毛色形成有关,e等位基因主要与红色毛色形成有关. |
| 关键词: 奶牛 毛色 MC1R 蛋白结构 |
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| 基金项目:国家“863”计划项目（2006AA1021D9,2007AA10Z169）；山东省农业科学院科研创新基金项目（2006YCX028）；山东省农业科学院青年科研基金项目（2006YQN038） |
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| Study on red coat color gene and prediction of protein structure in dairy cattle |
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| Abstract: |
| 【Objective】 The objective of this study was to study the nucleotide sequence of MC1R gene and predict its protein structure in dairy cattle,and investigate the molecular mechanism of red coat color formed.【Method】 The PCR-RFLP method was used to genotype the individuals.The bioinformatics and biotechnology software were used to predict the secondary structure.【Result】 The result showed that EE genotype was the dominant genotype in black and white herd while ee in red and white-herd.Secondary structure change caused by deletion mutation resulted in an earlier terminating of the translation,which led the formation of red coat color.【Conclusion】 The allele E was mainly related with black coat color while e with red. |
| Key words: dairy cattle coat color MC1R gene protein structure |
