| 摘要: |
| [目的]建立一种苦马豆素(Swainsonine,SW)的微量定量检测方法.[方法]以甲基-α-D-吡喃甘露糖苷(me-Gal,纯度99%)为内标,在柱温200℃、进样口温度300℃、氢火焰离子化检测器(FID)温度280℃、高纯氮气载气压力200 kPa条件下,对苦马豆素进行气相色谱分析,以SW进样质量浓度对SW和me-Gal峰面积的比值进行回归分析,得回归方程,并进行单点校正因子测定和加样回收试验.[结果]单点校正因子为1.067 8,在0.047～6mg/mL SW进样质量浓度对SW和me-Gal峰面积比值的线性关系良好.加样平均回收率为107.33%,变异黄芪地上部分SW含量为(39.25±0.16)mg/kg,茎直黄芪地上部分SW含量为(43.65±0.13)mg/kg.[结论]气相色谱内标法准确、重现性好,可作为苦马豆素的微量定量检测方法. |
| 关键词: 黄芪属 疯草 苦马豆素 气相色谱 内标法 |
| DOI： |
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| 基金项目:国家自然科学基金项目（30571315） |
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| Determination of Swainsonine in Astragalus locoweed by internal standard gas chromatography |
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| Abstract: |
| 【Objective】 The study established a method for determination of Swainsonine.【Method】 Swainsonine in Astragalus locoweed was determinated by mainternal standard gas chromatography with me-Gal as internal standard under the oven temperature 200 ℃,injection temperature 300 ℃,detector（FID） temperature 280 ℃ and flow rate of carrier gas 200 kPa.【Result】 The analysis results of single calibration-ratio was 1.067 8 and good linear relationship was obtained in the range of 0.047 to 6 mg/mL.The recovery percentage was 107.33％.The SW contents of A.variabilis and A.strictus were（39.2±0.16）mg/kg and （43.6±0.13）mg/kg respectively.【Conclusion】 The internal standard method had high accuracy and good repertability and could be a determination method of Swainsinine. |
| Key words: Astragalus locoweed Swainsonine gas chromatography internal standard method |
