| 摘要: |
| [目的]制备转MnSOD基因仙客来植株,检测其对高温胁迫的抗性.[方法]以仙客来组培苗的叶片为受体材料,采用根癌农杆菌介导法将MnSOD基因导人仙客来,对获得的抗性株系进行分子生物学检测,并测定转MnSOD基因仙客来组培苗在36℃高温胁迫下的SOD活性、MDA含量、细胞膜相对透性和可溶性蛋白含量.[结果]获得35个仙客来抗性株系,其中3个株系经GUS组织化学染色、PCR和Southern印迹检测表现为阳性,表明外源基因整合到仙客来基因组中.转基因仙客来的SOD活性始终高于非转基因植株,细胞膜透性和MDA含量上升的速度低于非转基因植株,可溶性蛋白含量高于非转基因植株.[结论]获得了转MnSOD基因仙客来植株,其对高温胁迫的抗性高于非转基因植株. |
| 关键词: 仙客来 遗传转化 GUS Southern 高温胁迫 |
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| 基金项目:教育部留学回国人员科研基金项目（2003-14）；西北农林科技大学“拔尖人才支持计划”项目 |
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| Transgenic Cyclamen plants expressing MnSOD gene and its resistance to high temperature stress |
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| Abstract: |
| 【Objective】 This study is to transform MnSOD gene to the Cyclamen plants,and to evaluate its effect on the endurance to high temperature stress and environmental stress.【Method】 With leaves as acceptor，MnSOD gene was incorporated into cyclamen through Agrobacterium tumefaciens mediated transformation.Resistant cyclamen plants were confirmed by molecular biological identification；SOD activity，MDA content，membrane permeability and protein content of high temperature stress at 36 ℃ of transformed cyclamen and non-transformed cyclamen were measured.【Result】 35 resistant plants were regenerated，3 of which were positive by GUS activity，PCR assay and Southern hybridization analysis.This result indicated that the MnSOD gene had integrated into cyclamen.The experimental data showed that the level of SOD activity in transformed cyclamen was higher than that in non-transformed plantlets；the membrane permeability and MDA content in transformed cyclamen rose more slowly than non-transformed plantlets；the protein content in transformed cyclamen was higher than that in non-transformed plantlets.【Conclusion】 The resistance of the transformed plantlets to high temperature stress was higher than non-transformed control. |
| Key words: Cyclamen persicum genetic transformation GUS Southern high temperature stress |
