| 摘要: |
| [目的]筛选适合于山羊胚胎移植的速眠新静脉注射麻醉剂量.[方法]以关中奶山羊为试验动物,速眠新Ⅱ注射液为麻醉剂,研究静脉注射麻醉时,不同剂量速眠新Ⅱ注射液对不同体重组(28～35,36～45和46～55 kg)山羊的麻醉效果;同时比较了速眠新Ⅱ注射液2种麻醉方法(静脉注射和肌肉注射)对山羊生理指标,心电图,麻醉山羊的诱导期、麻醉期、苏醒期,麻醉苏醒后山羊的采食、反刍及精神状况的影响.[结果](1)山羊胚胎移植受体静脉麻醉时,山羊体重为28～35,36～45及46～55 kg时,最适宜麻醉剂量分别为0.4,0.4和0.5 mL/只.(2)与麻醉前相比,麻醉后山羊的体温升高,呼吸次数和心率均降低.(3)2种麻醉方法下,麻醉效果优、良山羊的心电图均正常,无差异.(4)速眠新Ⅱ注射液静脉注射麻醉时,山羊的诱导期、麻醉期和苏醒期均较短,在苏醒后0.5 h,92.8%和87.5%的山羊可以采食和反刍,95.3%的山羊精神状况良好;苏醒后1 h基本上全部可以恢复正常.[结论]采用静脉注射麻醉山羊进行胚胎移植手术,速眠新Ⅱ注射液用药剂量小,且安全、有效,明显优于肌肉注射. |
| 关键词: 速眠新Ⅱ注射液 肌肉注射 静脉注射 麻醉 山羊 胚胎移植 |
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| 基金项目:陕西省重点攻关项目“干细胞研究与家畜胚胎工程示范”(2006kz05-G1) |
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| Application of SU-MIAN-XIN in goat's embryo transplant |
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| Abstract: |
| 【Objective】 The aim of this study was to screen the suitable dose of anesthesia intravenously for goats embryo transfer.【Method】 Anesthetic efficiency of different dose under vein injection in different weight（28－35 kg，36－45 kg and 46－55 kg）was compared,so were the two kinds of injection methods-intravenous injection and intramuscular injection using Su-Mian-Xin as the anesthetic injected to recipient goats.And the physiological index (body temperature,respiration,heart rate and electrocardiogram) and analepsia parameter (revulsiue duration,narcotism duration,resuming duration,pain reaction,foraging,rumination,and consciousness )were recorded.【Result】 (1) The optimal dose of anesthesia intravenously for receptor in goats embryo transfer were 0.4,0.4 and 0.5 mL of per-goat among three different weight groups 28－35 kg,36－45 kg and 46－55 kg respectively. (2) There were higher body temperature,lower respiratory frequency and heart rate compared with the pre-anesthetic stage.（3） Normal electrocardiogram of goats after anesthesia when two kinds of injection methods was used showing no difference.(4) The revulsiue duration,narcotism duration,resuming duration of goats by Su-Mian-Xin intravenous injection were shorter.After analepsia 0.5 h 92.8% and 87.5% of goats could forage and ruminate,and 95.3% were consciousness.And after analepsia 1 h,all could return to normal.【Conclusion】 Su-Mian-Xin anesthestic intravenous injection was safe and effective and the dose was obviously lower,and superior to intramuscular injection. |
| Key words: Su-Mian-Xin intramuscular injection intravenous injection anesthesia goat embryo transplant |
