| 摘要: |
| [目的]对狗舌草体积分数60% 乙醇提取物的毒理学安全性进行评价.[方法]利用C57L-L小鼠,以生理盐水为阴性对照,环磷酰胺为阳性对照,进行狗舌草体积分数60%乙醇提取物的90 d长期毒性试验、30 d蓄积试验.并采用小鼠骨髓微核试验、小鼠生殖机能试验和小鼠胎儿外观畸形检查,对狗舌草体积分数60% 乙醇提取物的长期毒性、蓄积毒性和特殊毒性进行评价.[结果]90 d长期毒性试验未见供试小鼠临床表现、体重和病理变化异常,30 d蓄积试验未见供试小鼠死亡,给药组与阴性对照组小鼠骨髓微核率无显著差异(P＞0.05);与阴性对照组相比,用药后受孕鼠的受孕率、每窝活胎数、平均胎儿重、吸收胎儿数和死胎数均无明显变化(P＞0.05),供试小鼠胎儿未见明显的外观畸形(P＞0.05).[结论]狗舌草体积分数60%乙醇提取物无长期毒性,无蓄积毒性,无致突变性和致畸胎性. |
| 关键词: 狗舌草 乙醇提取物 长期毒性 蓄积毒性 特殊毒性 小鼠 |
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| 基金项目:广东省自然科学基金项目（05011789）；广东省科技计划项目（2004B20201007） |
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| Studies on chronic toxicity, accumulation and special toxicities of extract from Tephroseris kirilowii (Turcz.) Holub |
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| Abstract: |
| 【Objective】 The study is to assess the toxicological safety of 60% (V/V) alcoholic extract from Tephroseris kirilowii Turcz Holub.【Method】 With a negative control of normal saline and a positive control of cyclophosphamide,the chronic toxicity,accumulation and special toxicities were assessed by employing a range of C57L-L mice to conduct the tests of 90 day chronic toxicity,30-day accumulation,bone-marrow micronucleus and reproduction ability as well as fetal malformation of the T.kirilowii extract.【Result】 There were no abnormalities in clinical exhibition,body weight and visible pathology in 90-day chronic toxicity test and no death in 30-day accumulation test.Being compared to the negative control group,the mouse bone-marrow micronucleus rate was not changed (P>0.05) and there were no significant changes (P>0.05)in numbers of live,absorbed and dead fetuses of the pregnant mice and no visible fetal malformation in the teratogenic test (P>0.05).【Conclusion】 The T.kirilowii extract with 60% (V/V) ethanol did not cause chronic toxicity,accumulation,mutagenicity and teratogenicity. |
| Key words: Tephroseris kirilowii (Turcz.) Holub ethanol extract chronic toxicity accumulation special toxicity mouse |
