| 摘要: |
| [目的]为建立醋酸甲羟孕酮(MPA)快速准确的免疫学分析技术,对MPA人工抗原的合成鉴定进行研究.[方法]将MPA肟化后与琥珀酸酐反应制得MPA半琥珀酸(MPA-HS),然后通过碳二亚胺法将MPA-HS与牛血清白蛋白(BSA)偶联合成人工抗原MPA-BSA,并采用紫外全波长扫描法、SDS-PAGE法、ELISA检测法和动物免疫试验对其进行鉴定.[结果]MPA与BSA的偶联比为8:1时,合成的人工抗原MPA-BSA具有良好免疫原性与反应原性.[结论]成功合成了人工抗原MPA-BSA,为进一步的MPA免疫学分析技术研究奠定了基础. |
| 关键词: 醋酸甲羟孕酮 半抗原 人工抗原 合成与鉴定 |
| DOI： |
| 分类号: |
| 基金项目:国家质检总局科研项目（2006IK017） |
|
| Synthesis and identification of artificial antigen for Medroxyprogesterone Acetate |
|
|
| Abstract: |
| 【Objective】 This study was to develop a rapid and sensitive technology for the detection of Medroxyprogesterone Acetate（MPA）.The atrificial antigen of MPA was synthesized and identified in the study.【Method】 MPA hemi hemi-succinate (MPA-HS) was produced by the oximation reaction of MPA with the reaction between oximinooximated-MPA and with succinic anhydride,then the hapten MPA-HS was conjugated to protein carrier BSA by using the coupling agent diisopropylcarbodiimide,and the synthesis synthesized antigen MPA-BSA was identified by full-wavelength the ultraviolet full wavelength scanner,SDS-PAGE,ELISA test and animal-inoculation immunization test.【Result】 The results showed that the synthesis synthesized antigen MPA-BSA with good immunogenicity and reactogenicityantigenicity,and the optimized conjugation molecular ratio of coupling reaction was determined to be 8∶1.【Conclusion】 The synthesis of artificial antigen MPA-BSA set basis for the study of the immunology detection technology of MPA. |
| Key words: Medroxyprogesterone Acetate hapten artificial antigen synthesis and identification |
