| 摘要: |
| 为了寻找影响奶牛产奶性状的候选基因，采用PCR-SSCP分子标记方法,对中国荷斯坦奶牛LOX-1基因3′非编码区进行了多态性分析。结果表明，在中国荷斯坦奶牛中检测的LOX-1基因3′UTR有AA型、BB型和AB型3种基因型，其频率分别为0.137 4，0.313 0和0.549 6；不同基因型与中国荷斯坦奶牛的乳脂率之间有显著的相关性（P<0.05），但对乳蛋白率、产奶量和体细胞评分（SCS）影响不大，未达到显著水平。初步认为LOX-1基因可能是控制中国荷斯坦奶牛产奶性能的一个候选基因，或是与之存在紧密遗传连锁的分子标记。 |
| 关键词: 中国荷斯坦奶牛 LOX-1基因 PCR-SSCP |
| DOI： |
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| 基金项目:国家“十五”科技攻关计划奶类重大专项 (2002BA518A17)；陕西省科学技术研究发展计划项目(2004K01-G1) |
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| Study on the 3′UTR of LOX-1 gene in Chinese Holstein by PCR-SSCP |
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| Abstract: |
| In order to detect polymorphisms in the lectin like oxidized low density lipoprotein receptor 1(LOX-1) gene,the polymorphism of LOX-1’s 3′UTR in Chinese Holstein was detected through PCR-SSCP (Polymerase Chain Reaction Single Strand Conformation Polymorphism).The result showed that three genotypes AA,BB and AB of the 3′UTR of LOX-1 gene were found,and the genotype frequency were 0.137 4，0.313 0，0.549 6,respectively in Chinese Holstein.There was highly significant correlation between different genotypes in fat content（P<0.05）.No significant effect of different genotypes on their ME,protein content and SCS (Somatic Cell Score) was found.These results preliminarily showed that the LOX-1 gene was either a candidate gene that influenced the prolificacy in Chinese Holstein or a molecular marker in close linkage with such a gene. |
| Key words: Chinese Holstein LOX-1 gene PCR-SSCP |
