| 摘要: |
| 利用抗真菌病害基因转化苹果主栽品种“嘎拉”,以期提高其对真菌病害的抗性。通过对影响农杆菌介导苹果遗传转化因素的研究,建立了农杆菌介导的嘎拉叶片遗传转化体系。利用叶盘法转化受体材料,将-β1,3-葡聚糖酶基因及几丁质酶基因导入苹果中。对获得的"嘎拉"转化植株进行PCR检测,结果初步表明,外源基因已经整合到"嘎拉"苹果基因组中。 |
| 关键词: 苹果 抗真菌病害基因 农杆菌介导 遗传转化 |
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| 基金项目:陕西省重大科技专项（2006kz05-G4）；西北农林科技大学“拔尖人才支持计划”项目 |
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| Study on transformation of apple with antifungal gene |
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| Abstract: |
| The objective of the present research was to introduce genes with antifungal potential into the commercially important apple cvs.Gala in order to improve its resistance against fungal diseases.By studying factors affecting Agrobacterium mediated genetic transformation,the Agrobacterium mediated genetic transformation system of Gala was established.And the β-1,3-glucanase and chitinase genes were introduced into Gala by means of leaf discs and finally the objective seedlings of Gala was obtained.The transformation plants were tested by PCR detection and the result showed that the exogenous genes had been introduced into the regeneration plants genome. |
| Key words: apple(Malus domestica Borkh.),antifungal gene,Agrobacterium-mediated,genetic transformation |
