| 摘要: |
| 为了揭示紫外线诱导桃蚜的生态学及分子变异的遗传效应,以在正常环境中生长的桃蚜为对照,研究了紫外线不同辐射强度（15,30,45 W）和时间（2,6 h）对桃蚜（Myzus persicae）生态学参数以及线粒体基因COⅠ-Ⅱ突变的影响。结果表明,不同辐射处理对桃蚜F2代的主要种群参数均有不同程度的影响：当辐射时间为2 h时,紫外线强度为15 W处理的桃蚜种群内禀增长率最高,达0.265;当辐射时间为6 h时,内禀增长率随紫外线辐射强度的增加而缓慢上升。与对照相比,低强度（15 W）紫外线处理桃蚜的平均世代周期和净增殖率较其他处理明显增加。对紫外线诱导桃蚜线粒体基因COⅠ-Ⅱ进行测序可知,15 W紫外线辐射2 h处理桃蚜的线粒体基因COⅠ-Ⅱ发生单碱基突变,而45 W紫外线辐射6 h处理桃蚜的线粒体基因COⅠ-Ⅱ突变位点达9个。说明紫外线诱导对当代桃蚜的生态学和分子变异的影响已经遗传给了后代。 |
| 关键词: 紫外线诱导 桃蚜 生态学 线粒体基因COⅠ-Ⅱ |
| DOI： |
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| 基金项目:国家自然科学基金项目（39970112，30470268）；陕西省重点项目（2001SM01） |
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| UV-induced ecological response and COⅠ-Ⅱ mutation of Myzus persicae |
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| Abstract: |
| In order to reveal UV-induced ecological and molecular mutational genetic effects of Myzus persicae,the biological response and COⅠ-Ⅱ mutation were observed compared with the normal control after different treatments，including time and intensity (15,30,45 W)) time (2,6 h).The results indicated that based on the life-fecundity table of irradiation population in labs,the ecological parameters changed with the time and intensity;after two hour UV irradiation,the intrinsic rate reached the top,0.265;after six-hours UV irradiation,the intrinsic rates showed the increasing tendency with the strenghtening of ultraviolet radiation;in addition,compared with the control,the other parameters increased obviously.According to mtDNA COⅠ-Ⅱ sequences,there was only 1 site producing mutation for the treatment (15 W-2 h),while as for the other treatment (45 W-6 h) 9 variable sites were found. Moreover,this work demonstrated that the ecological and gene mutation had been delivered to the progeny. |
| Key words: ultraviolet radiation Myzus persicae ecology mitochondrial DNA (mtDNA) COⅠ-Ⅱ |
