| 摘要: |
| 为了确定山茱萸的抑菌活性成分,以几种常见的食品微生物为供试菌种,采用生物活性追踪法对山茱萸抑菌活性成分进行了初步分离与鉴定。结果表明,山茱萸体积分数70%乙醇粗提液和乙酸乙酯萃取物对供试细菌、霉菌均有较好的抑菌活性,对酵母菌的抑菌活性不明显;利用大孔吸附树脂柱层析和薄层层析对抑菌活性较强的乙酸乙酯组分进一步分离,得到2个活性组分,经化学定性检识,初步判定山茱萸抑菌活性成分为酚酸类化合物。 |
| 关键词: 山茱萸 抑菌活性 分离检测 有效成分 食品防腐剂 |
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| Studies on extraction and isolation of bacteriostatic active composition from Fructus Corui |
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| Abstract: |
| To study the antibacterial activity of extracts from Fructus Corui,antimicrbial activities of Fructus Corui extracts with ethanol against food spoilage were tested in this paper.Its functional compositions were preliminarily identified.The result showed that the ethyl acetate extract of Fructus Corui extracted by 70% ethanol showed stronger antifungal activities against bacteria and mold,but insidiously against yeast;two fractions were researched after separation by macroporous adsorption resin and silica-gel thin layer chromatograph,and the chemically identified results showed that the bacteriostatic active eluates were composed of phenolic acid. |
| Key words: Fructus Corui,bacteriostatic activity,extract and isolate,effective components,food preser vative |
