| 摘要: |
| 为了确定木瓜蛋白酶、胰蛋白酶、枯草杆菌蛋白酶酶解鲤鱼的最佳酶解条件及其酶解效果,应用正交试验和对比试验对鲤鱼酶解液化技术进行了研究,并对酶解前后游离氨基酸含量进行了分析。结果表明,胰蛋白酶酶解鲤鱼的最佳酶解条件为：酶用量24 g/kg,酶解温度50 ℃,pH值7.0,在此条件下酶解3 h后氨基态氮含量为59.2 mg/L;枯草杆菌蛋白酶的最佳酶解条件为：酶用量0.16 g/kg,酶解温度45℃,pH值8.0,在此条件下酶解4 h后氨基态氮含量为44.6 mg/L;木瓜蛋白酶的最佳酶解条件为：酶用量10 g/kg,酶解温度60 ℃,pH值7.0,在此条件下酶解4 h后氨基态氮含量为102.6 mg/L。枯草杆菌蛋白酶酶解鲤鱼后,游离氨基酸含量由185.91 mg/L增加到779.91 mg/L,增加了76.16%。通过对3种蛋白酶在最佳条件下酶解鲤鱼的比较可知,枯草杆菌蛋白酶酶解效果较好。 |
| 关键词: 鲤鱼 酶解 胰蛋白酶 木瓜蛋白酶 枯草杆菌蛋白酶 氨基态氮 |
| DOI： |
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| 基金项目:西北农林科技大学科技创新基金项目 |
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| Technology reseach of enzymatic hydrolysis for the muscle of carp |
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| Abstract: |
| The optimum conditions of trypsin,subtilisin,and pepsin enzymatic hydrolysis for carp was studied,and the effect of enzymatic hydrolysis was compared.Orthogonal experiment and comparative research were used to study enzymatic hydrolysis conditions of carp,and analyze the content of free Amino acids before and after enzymatic hydrolysis for carp.The optimum conditions for the trypsin enzymatic hydrolysis were enzyme concentration 24 g/kg,enzymatic temperature 50 ℃，and pH 7.0.Under this condition the content of amino nitrogen was 59.2 mg/L after 3 hours of enzymatic hydrolysis.The optimum conditions for the Subtilisin were enzyme concentration 0.16 g/kg,enzymatic temperature 45 ℃,pH8.0,and the content of amino nitrogen was 44.6 mg/L after 4 hours.The optimum conditions for the Pepsin Enzymatic hydrolysis were enzyme concentration 10 g/kg,enzymatic temperature 60 ℃,pH 7.0,and the content of amino nitrogen was 102.6 mg/L after 4 hours of enzymatic hydrolysis.After subtilisin enzymatic hydrolysis for carp,the content of free Amino acids increased from 185.91 mg/L to 779.91 mg/L，increasing by 76.16％.Through comparing the three protease enzymatic hydrolysis for carp,subtilisin was the better protease for carp. |
| Key words: carp enzymatic hydrolysis trypsin pepsin subtilisin amino nitrogen |
