| 摘要: |
| 采用紫外分光光度法对24份不同类型硬粒小麦的类胡萝卜素含量进行了测定,建立了准确、快速和简便的硬粒小麦类胡萝卜素含量测定方法。结果表明,不同硬粒小麦品种类胡萝卜素光谱都显示β-胡萝卜素特征吸收光谱峰形,特征吸收峰在425～478 nm,主峰在445 nm,确定的测定波长为445 nm;不同硬粒小麦品种类胡萝卜素含量差异达极显著水平,变异范围在3.533 3～8.653 3 mg/kg,平均值为5.886 mg/kg,其中墨西卡里75类胡萝卜素含量最高,为8.653 3 mg/kg,其次是CD85007-15-2、P468、新九师89-1、M75、中硬86173I、D84001、85(363)6,含量为6.573 3～7.480 0 mg/kg,类胡萝卜素含量极低的硬粒小麦品种有可可瑞特和P163。 |
| 关键词: 硬粒小麦 类胡萝卜素 定量分析 |
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| 基金项目:农业部“948”项目“优质抗病小麦种质的引进、评价、改良与创新”（2003-Q01）;国家“863”项目“优质抗病小麦分子育种技术与应用研究”（2002AA207003）；陕西省攻关项目“小麦生物技术育种及产业化开发”（2003K03-G1-01） |
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| Study on quantitative analysis of total carotenoids content in triticum durum |
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| Abstract: |
| Carotenoids were extracted from triticum durum seeds in this experiment.The content of total carotenoids of different varieties of triticum durum was studied by using the ultraviolet-visible spectrophotometry.The result showed that the UV-visible spectra apexes of β-carotene was all showed on the carotenoid spectra of different triticum varieties.The apexes are on 425-478 nm and the main peak is on 445 nm,and the identified wavelength is 445 nm.Significant differences in carotenoids content were found among different varieties.The carotenoids content of 24 varieties varied from 3.533 3-8.653 3 mg/kg, and the average was 5.886 mg/kg；the highest content variety was Moxikali75 which was 8.653 3 mg/kg.The next content varieties were CD85007-15-2,P468,Xinjiushi 89 1,M75,Zhongying 86173,ID84001,85（363）6 and the lowest content varieties were Kekeruite and P163 |
| Key words: triticum durum,carotenoids,quantitative analysis |
