| 摘要: |
| 采用超纳滤技术浓缩家兔产气荚膜梭菌(A)型疫苗,建立家兔产气荚膜梭菌(A)型疫苗浓缩工艺。配制家兔产气荚膜梭菌(A)型超纳滤浓缩疫苗,并对其无菌性、安全性和免疫效力进行了检验。结果显示,该工艺可将家兔产气荚膜梭菌(A)型灭活抗原浓缩约7倍,超纳滤膜用NaOH洗后其通量可以恢复;配制的家兔产气荚膜梭菌(A)型超纳滤浓缩疫苗无菌检验合格,安全检验家兔无不良反应,符合动物用生物制品要求;以2 mL/只的剂量免疫家兔,家兔产气荚膜梭菌(A)型超纳滤浓缩疫苗的保护率达100%,传统铝胶疫苗对照组的保护率平均为86.7%,表明超纳滤浓缩疫苗免疫效力优于传统铝胶疫苗。确定了家兔产气荚膜梭菌病(A)型疫苗超纳滤膜膜浓缩的工艺。 |
| 关键词: 家兔 产气荚膜梭菌 膜浓缩 免疫效力 |
| DOI： |
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| 基金项目:江苏省科技成果转化专项（BA2004032） |
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| Techniques of ultrafiltration membrane concentration of inactive vaccine against clostridium perfringens type A of rabbits |
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| Abstract: |
| Studies were made on concentration of inactive vaccine against clostridium perfringens type A of rabbits by ultrafiltration technology,and techniques of ultrafiltration membrane concentration were established.The antigen was condensed 7 times by ultrafiltration membrane.The vaccine made of the condensed antigen was measured safe and effective by asepsis.Results showed that the flux of filter could be resumed after the use of NaOH.The aseptic examination was qualified,and the rabbits behaved well without any bad reaction by safe examination,Besides,the vaccine against clostridium perfringens could provid 100% of protection when injected with 2 mL vaccine,in contrast,the vaccine of control group provided 86.7% of protection,which showed that the vaccine produced by ultrafiltration membrane was better than traditional aluminium vaccine.So the technique of ultrafiltration membrane clostridium perfringens type A is confirmed. |
| Key words: rabbits,clostridium perfringens,ultrafiltration membrane concentration,mensuration of effectiveness |
