| 摘要: |
| 将千里光全草粉用体积分数70％乙醇浸泡，经超声波-微波处理，用乙醚萃取脱色，冷冻真空干燥，制备千里光提取物冻干粉（SCE）。以对乙酰氨基酚为对照，采用醋酸扭体法和热板法研究不同剂量SCE的镇痛作用；采用骨髓微核试验研究不同剂量SCE的致突变作用。结果发现，122.72 mg/kgSCE具有显著的镇痛作用，而130.90 mg/kg SCE对雌雄小白鼠均不具有致突变作用。说明122.72～130.90 mg/kg SCE能同时满足无致突变作用和显著镇痛作用的双重条件。 |
| 关键词: 千里光提取物 镇痛作用 微核试验 致突变作用 小鼠 |
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| 基金项目:广东省科技攻关计划重点引导项目(2004B20201007)；湛江科技攻关计划项目(湛科［2006］94号) |
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| Analgesic effect of Senecio scandens extract and its mutation test in mice |
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| Abstract: |
| By marinating ground whole Senecio scandens Buch-Ham with 70%(V/V) of ethanol solution,treating it with ultrasonic-microwave,and decoloring it with ether,then drying it with frozen vacuum,thus the frozen-vacuum-dried powder of S.scandens extract(SCE) was prepared.The analgesic effects of SCE were conducted with the hot-plate test and acetic acid writhing method with a reference of acetaminophen,respectively.And the bone-marrow micronucleus test was conducted to assess the mutagenic effect of SCE.The results showed that SCE at 122.72 mg/kg significantly exerted analgesic effects,while 130.90 mg/kg of SCE did not cause mutagenic effect neither in male nor in female mice.The results above indicated that SCE at 122.72 mg/kg to 130.90 mg/kg can at the same time meet two requirements of performing significant analgesic effects and no mutagenic effect in mice. |
| Key words: Senecio scandens Buch.-Ham. extract analgesic effect bone-marrow micronucleus test mutation mouse |
