| 摘要: |
| 分别从陕西咸阳、兴平、杨凌、柔谷、眉县、岐山、凤翔等地采集辣椒病毒病标样126份，在室内通过单斑分离及回接验证得到5种分离物，采用鉴别寄主的生物学反应和DAS-ELISA法鉴定，结果表明，引起陕西辣椒病毒病的毒原有黄瓜花叶病毒（CMV）、烟草花叶病毒（TMV）、烟草蚀纹病毒（TEV）、马铃薯Y病毒(PVY)和蚕豆萎蔫病毒（BBWV），其中CMV和TMV是优势毒原种群，分别占检测样品的60.31%和30.94%。在室内分别以CMV和TMV的枯斑寄主苋色藜和心叶烟为测试寄主，采用半叶法对接种叶片分别于接种前和接种后涂施病毒抑制剂，测试了7种病毒抑制剂的抑制效果。结果表明，接种前后涂施3.85%病毒必克水乳剂500倍液,均对黄瓜花叶病毒和烟草花叶病毒有很好的防治效果，且接种前涂施的防治效果较接种后涂施的防治效果好。 |
| 关键词: 辣椒病毒病 毒原鉴定 病毒抑制剂 防治效果 |
| DOI： |
| 分类号: |
| 基金项目:西北农林科技大学创新团队资助项目 |
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| Virus identification and screening virusicides of pepper virus disease in Shaanxi Province |
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| Abstract: |
| 126 typical samples of viral pathogens in pepper were collected from Xianyang,Xingping,Yangling,Rougu,Meixian,Qishan,Fengxiang and so on in Shaanxi province respectively.These samples were identified by biological reactions of different host plants and DAS ELISA.The results showed that pepper in Shaanxi province were infected with TMV,CMV,TEV,PVY and BBWV,and the most predominant viruses were CMV and TMV,the proportion being 60.31% and 30.94% respectively.Inhibiting effect of several plant virus inhibitors against CMV and TMV were tested in laboratory.TMV was inoculated with Nicotiana glutinosa,CMV with Chenopodium amaranticola,then these plant virus inhibitors were smeared over the half leaf before inoculation and 24 hours after inoculation.The results showed that the efficacy of 3.85% Bilken virusicide was the best to TMV and CMV when diluted 500 times either before or after inoculation.And the control effect before inoculation was better than that after inoculation. |
| Key words: pepper virus disease virus identification plant virus inhibitor control effect |
