| 摘要: |
| 为了探讨促性腺激素释放激素（gonadotropin releasing hormone,GnRH）在青年奶山羊下丘脑的表达特点，采用灵敏度较高的免疫组化SP法研究了GnRH神经元在6～8月龄青年奶山羊下丘脑中的分布。结果表明，分泌GnRH的神经元主要分布在视上核、下丘脑前核、室旁核和弓状核，其次是视前内侧核、视前外侧核、交叉上核、下丘脑外侧区、视上弥散核、腹内侧核、腹外侧核及乳头体各核团,前连合核、终纹床核、穹窿周核、环核、视前交叉上核和背内侧核分布较少。GnRH阳性神经元的形态有圆形、三角形、卵圆形、梭形、多角形、不规则形，有些阳性神经元还具有明显的突起;圆形和三角形细胞直径为7～27 μm，卵圆形和梭形细胞短轴和长轴分别为7～21和10～48 μm;GnRH阳性神经纤维仅见于视前内侧核、视前外侧核、弓状核、乳头体后核、乳头体内侧核等核团,在正中隆起和第三脑室室周也可见中等数量的阳性纤维。结果提示，GnRH神经元在青年奶山羊下丘脑分布广泛。 |
| 关键词: 下丘脑 GnRH神经元 免疫组织化学 青年奶山羊 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（30170683） |
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| Distribution of gonadotropin releasing hormone neuron in the hypothalamus of youth dairy goat |
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| Abstract: |
| To study expressive characteristics of gonadotropin releasing hormone (GnRH) in the hypothalamus of youth dairy goat,the distribution of GnRH neuron in the hypothalamus of youth dairy goat was determined by immunohistochemical streptavidin-peroxidase (SP) method.The results revealed that GnRH-immunoreactive (GnRH-IR) neurons were mainly distributed over nucleus supraopticus,nucleus anterior hypothlomi,nucleus paraventricularis and nucleus arcuatus;in addition,a certain GnRH-IR neurons were also found in nucleus preopticus medialis,nucleus preopticus lateralis,nucleus suprachiasmaricus,nucleus lateralis hypothalami,nucleus supraopticus diffusus,nucleus ventrodialis,nucleus ventrolateralis and nucleus mamillaris;and the rest were found to distribute over nucleus anterior commissuris,bed nucleus stria terminalis,nucleus perifornicalis,nucleus circularis,nucleus preopticus suprachiasmaticus and nucleus ventromedialis.The positive cells were mainly circular,triangle,oval,spindle-shaped,polygon,irregular shape,in addition,some of them had obvious projects.The circular and the triangle cell diameters were 7-27 μm,the oviod and the spindle-shaped cell minor axis and the majoraxis were 7-21 μm,10-48 μm respectively.GnRH-IR nerve fibers were only observed in nucleus pre-opticus medialis,nucleus preopticus lateralis,nucleus paraventricularis and nucleus suprachiamaticus.Also,some nerve fibers were found in medium eminence and the surrounding area of third ventricle.The results showed that the distribution of GnRH in the hypothalamus of youth dairy goat was extensive. |
| Key words: hypothalamus GnRH immunochemistry young dairy goat |
