| 摘要: |
| 一些低丰度的miRNA和组织特异性miRNA往往很难发现，利用生物信息学方法，根据已知水稻miRNA的各种属性，设计miRNA前体预测程序—PreMiRFind，从全基因组范围中预测出1375条miRNA前体，然后利用已知拟南芥的miRNA对这1 375条前体进行同源检索，找出166条miRNA前体，再用水稻已知的miRNA对这166条序列进行检索，发现其中153条与已知的水稻microRNA完全相同，对剩余的13条miRNA前体预测序列用mFold作进一步结构分析，最后得到10条新的候选miRNA基因序列。 |
| 关键词: 水稻基因组 |
| DOI： |
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| 基金项目:国家转基因研究与产业化开发专项(JY03A-11-01)；陕西省农业分子生物学重点实验室项目 |
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| Computational identification of novel family members of microRNA genes in Oryza sativa genome |
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| Abstract: |
| MicroRNAs(miRNA) are about 21 nt long non-coding RNAs derived from larger hairpin RNA precursors and play important regulatory roles in both animals and plants.The low abundance of some miRNAs and their time-and tissue-specific expression patterns make experimental miRNA identification difficult.Here a program-PreMiRFind for genome-wide prediction of Oryza microRNAs was presented.This method used characteristic features of known Oryza miRNAs as criteria to search for miRNAs.Our prediction identified 1 375 pre-miRNA candidates.The 166 premiRNAs were reservation as criteria to search for miRNAs conserved between Arabidopsis and Oryza sativa.153 pre-miRNAs were the same as known miRNAs and then the structures of the 13 pre-miRNAs were analyzed further.Finally 10 new miRNAs were obtained. |
| Key words: rice genome RNAfold microRNA |
