| 摘要: |
| 采用活性跟踪法从紫花地丁的氯仿萃取物中分离得到1个抗菌活性成分(化合物1),并以停乳链球菌、金黄色葡萄球菌、大肠杆菌、无乳链球菌、乳房链球菌和沙门氏菌为供试菌,对紫花地丁的抑菌活性及其活性成分进行了初步研究。结果表明,化合物1初步鉴定为黄酮类化合物;紫花地丁的主要活性成分位于甲醇-水(V(甲醇)∶V(蒸馏水)=1∶1)萃取物和氯仿萃取物中;化合物1对以上6种供试菌的最小抑菌浓度依次为0.039,0.078,0.156,0.313,0.625和1.25 mg/mL;对金黄色葡萄球菌、无乳链球菌和乳房链球菌的最小杀菌浓度≤2.5 mg/mL,对停乳链球菌、沙门氏菌和大肠杆菌的最小杀菌浓度>2.5 mg/mL。 |
| 关键词: 紫花地丁 抗菌活性 抗菌成分 抗菌活性跟踪 分离纯化 |
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| 基金项目:陕西杨凌科研开发基金项目(2003JB03-1) |
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| Antibacterial constituent of Viola yedoensis Makino |
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| Abstract: |
| Antibacterial activity of Viola yedoensis Makino and its antibacterial constituents were studied in this paper against S.aureas,S.agalactiae,S.uberis,S.dysagalactiae,E.coli and Salmonella in vitro.One antibacterial constituent (compound 1) from the chloroform extract was obtained with tracing antibacterial activity method.The results showed that compound 1 was preliminarily identified as flavone;that main antibacterial comstituents in V.yedoensis Makino existed in chloroform phase and ethanol water (1∶1) phase,that minimal inhibitory concentrations (MIC) of compound 1 were 0.039,0.078,0.156,0.313,0.625 and 1.25 mg/mL respectively against S.dysagalactiae,S.aureas,E.coli,S.agalactiae,S.uberis and Salmonella,and minimal bactericidal concentrations (MBC) were ≤2.5 mg/mL against S.aureas,S.agalactiae and S.uberis,and >2.5 mg/mL against S.dysagalactiae,E.coli and Salmonella. |
| Key words: Viola yedoensis Makino antibacterial activity antibacterial constituent tracing-antibacterial activity isolation and purification |
