| 摘要: |
| 以灭活大肠杆菌(E.coli) O157为抗原免疫产蛋鸡获得抗E.coli O157卵黄抗体(IgY),建立了该抗体的间接酶联免疫吸附试验(ELISA)检测方法。并研究了包被温度和时间及不同卵黄前处理方法对ELISA检测的影响,所获得的最佳检测条件为:以浓度为108～109/mL的灭活E.coli O157为抗原进行包被,4 ℃包被19 h,水稀释法对卵黄进行前处理,然后检测其中的IgY效价。该方法简便可靠,适于对大肠杆菌特异性抗体IgY的动态检测和批量检测。 |
| 关键词: 大肠杆菌O157 卵黄抗体 酶联免疫吸附试验 |
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| 基金项目:陕西科技大学自然科学基金项目(ZX05-25);陕西科技大学创新团队项目(SUST-B12) |
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| Indirect ELISA detection of IgY specific on E.coli O157 |
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| Abstract: |
| Chicken was immunized with E.coli O157 as antigen,which produced a large amount of immunoglobulin of high titer in egg yolk,also called IgY.Indirect ELISA detection of IgY specific on E.coli O157 was studied.The parameters were as follows:polystyrene chloride microtitration plates were coated with E.coli O157 antigen at the amount of 108-109/mL，19 h at 4 ℃.The working concentration of HRP-labelled rabbit antichicken IgG was 1∶320.Egg yolk pretreated with water-dilution improved the performance of ELISA detection.The ELISA system is dependable and good for the assay and surveillance of E.coli O157 specific IgY. |
| Key words: Escherichia coli O157 immunoglobulin of yolk(IgY),enzyme-linked immunosorbent assay |
