| 摘要: |
| 以中国西门塔尔牛肝脏组织为材料，运用同源序列克隆技术并结合RT-PCR技术，对牛ACAS2基因的部分cDNA进行了克隆与序列分析，应用SUNbRH7000型辐射杂种板对分离的牛ACAS2基因进行了染色体定位。结果显示，本研究所获得的长为1535bp的cDNA序列为牛ACAS2基因的部分编码序列，该段序列与人（GenBank登录号：NM139274）和小鼠（GenBank登录号：NM019811）的ACAS2基因mRNA序列的相似性分别为91％和88％；牛ACAS2基因被定位于牛的13号染色体上，与该染色体上的标记DIK43513的距离为1.51cR。 |
| 关键词: 牛 乙酰辅酶A合成酶2基因 基因克隆 辐射杂种板 染色体定位 |
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| 基金项目:国家高技术研究发展计划项目（863计划）“优质鲁西黄牛新品系选育技术研究”（2002AA242011） |
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| Study on the cloning,sequence analysis and chromosomemapping of bovine ACAS2 gene |
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| Abstract: |
| The liver tissue from Chinese Simmental cattle was collected to extract RNA and the cDNA of the bovine ACAS2 gene was determined and analyzed by homology cloning approach combined with RT-PCR in this study.Sequence analysis and bioinformatics study showed that this partial cDNA contained 1 535 nucleotides.And its basic composition showed 91% and 88% identity with mRNA sequence,ACAS2 gene of the human NM_-13274 and mouse NM_-019811 respectively.The SUNbRH panel was employed to determine the precise location of both three genes.Statistical analysis revealed that,the bovine ACAS2 gene was mapped to the bovine chromosome(BTA) 13 and was closely linked to a framework marker DIK4350 at a 2-point LOD score of 36.4. |
| Key words: cattle ACAS2 gene cloning RH chromosome mapping |
