| 摘要: |
| 为了探讨γ-干扰素（interferon-γ，IFN-γ）在下丘脑-垂体-性腺轴（hypothalamic-pituitary-go-nadal axis，HPG轴）中的免疫调节作用，应用免疫组织化学SP法对IFN-γ免疫反应阳性产物在怀孕早期山羊HPG轴的分布特点进行了研究。结果显示，IFN-γ分布于乳头体内侧核、视前内侧核、下丘脑前区、腹内侧核、连接核、室旁核、视上核等20个核团中，各部分核团细胞着色程度深浅不一；在视上核、室旁核分泌部、乳头体内侧核等有大量的阳性神经纤维；同时在室周核、视前外侧核、视前内侧核等13个核团中散布有一些阳性星形胶质细胞，与阳性神经细胞交错共存；腺垂体中腺细胞和神经垂体中神经纤维均有IFN-γ表达；卵泡和黄体中也有IFN-γ存在。表明IFN-γ与动物的神经内分泌活动关系密切，参与了免疫-神经-内分泌网络中的生殖免疫调节。 |
| 关键词: γ-干扰素 HPG轴 怀孕早期 奶山羊 免疫组织化学SP法 |
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| 基金项目:国家自然科学基金项目（30170683）；陕西省自然科学基金项目（2002C104） |
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| Expression of IFN-γ immunoreactivity on HPG axis of dairy goat during early pregnancy |
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| Abstract: |
| To probe into the immunomodulation of IFN-γ to hypothalamic-pituitary-gonadal axis,the distribution of IFN-γ immunoreactivity on HPG axis of dairy goat during early pregnancy was determined by SP method of immuno-histochemistry.The result showed that IFN-γ was distributed over 20 nucleus of the hypothalamus,such as nucleus mamillaris medialis,nucleus pre-opticus medialis,area anterior hypothalami,nucleus ventromedialis,nucleus reunions,nucleus paraventricularis,nucleus supra optic,and etc.Many of IFN-γ positive nerve fibers were found in supra-optic nucleus,paraventricular nucleus,nucleus mamillaris medialis and etc.Nerve fibers and astrocytes IFN-γ immunoreactive positive cells spreaded in periventricular nucleus,nucleus pre-opticus lateralis,nucleus pre-opticus medialis and etc.At the same time,IFN-γ is also observed obviously in adenohypophysis and neurohypophysis.IFN-γ also expresses in follicular and corpus luteum.The results suggest that IFN-γ is related to the activity of neuroendocrine of animals,and is concerned with the reproductive immunity regulation in immuno-neuro-endocrine network. |
| Key words: IFN-γ HPG axis early pregnancy dairy goat SP method |
