| 摘要: |
| 采用RT-PCR技术克隆了水稻O-甲基转移酶ZRP4基因的cDNA片段，并进行了序列测定与分析。结果表明，该序列全长1 101 bp，编码366个氨基酸，与GenBank中水稻ZRP4基因序列比对，核苷酸同源性为99.8％，氨基酸同源性为99.7％。将克隆片段插入中间载体pBPFΩ7，经HindⅢ酶切回收带有P35s启动子和nos终止子片段，连接pCAMBIA1301载体，成功构建ZRP4基因的植物表达载体。 |
| 关键词: 水稻 ZRP4基因 基因克隆 植物表达载体 |
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| 基金项目:中国科学院知识创新工程重要方向项目（KZCX3-S-444）;教育部重点项目“01102 培育转耐盐基因水稻的研究” |
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| Cloning salt-tolerance related gene ZRP4 of rice and construction of its plant expression vector |
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| Abstract: |
| Utilizing RT-PCR,the cDNA of fragment of O-methlytransferanse ZRP4 gene in rice was successfully amplified,which was about 1 101 bp in length and encoded 366 amino acids,and was cloned and sequenced.DNA sequence analysis indicated that the cloned fragment shared high homogeneity to the cDNA of ZRP4 gene in rice.The cloned cDNA of ZRP4 gene was introduced into a middle vector pBPFΩ7.The fragment with P35s promoter and nos were digested by Hind Ⅲ, which were ligated into pCAMBIA1301 vector,giving an plant expression vector containing ZRP4 gene. |
| Key words: Oryza sativa L. ZRP4 gene gene cloning plant expression vector |
