| 摘要: |
| 以东方百合‘Siberia’试管苗为材料，研究了激素种类及其质量浓度、蔗糖浓度、大量元素浓度、低温处理对试管鳞茎形成和膨大的影响。结果表明，低质量浓度NAA有利于提高结鳞茎率；90 g/L蔗糖处理结鳞茎效果最好，所结鳞茎的平均直径为1.59 cm，平均鲜重为2.09 g，但与70 g/L蔗糖处理差异不显著；增加大量元素浓度可以促进鳞茎的形成和膨大，当浓度为2MS时，鳞茎直径最大达2.06 cm，平均直径1.65 cm，平均鲜重2.68 g，较处理前的单芽增加了12.8倍；5 ℃低温处理可使试管苗100％结鳞茎，处理30 d对结鳞茎的效果最好，鳞茎的直径、鲜重平均分别为1.50 cm，2.06 g。 |
| 关键词: 东方百合 试管鳞茎 组织培养 |
| DOI： |
| 分类号: |
| 基金项目:农业部新技术引进项目(2005-Z39) |
|
| Research on the growth of oriental lily bulblet in tube |
|
|
| Abstract: |
| The effect of the category and the concentration of hormone，the concentration of sugar and macro element,low temperature on bulblet formation and swelling were researched using plantlet of Oriental lily ‘Siberia’ in tube.The results showed that lower concentration of NAA can increase the bulblet formation rate.Sucrose can promote bulblet formation and swelling,and the best concentration was 90 g/L,mean diameter of the bulbet 1.59 cm,mean weight 2.09 g.Increasing the concentration of macroelement was favorable for bulblet formation and swelling.When the concentration was at 2 MS,the diameter of the biggest bulblet was 2.06 cm,mean diameter 1.65 cm,mean weight 2.68 g,the bulblet was 12.8 times bigger than the plantlet.5 ℃ treatment can make 100% plantlet form bulblet,and 30 days of treatment was best. |
| Key words: oriental lily bulblet in tube tissue culture |
