| 摘要: |
| 比较了在冷冻保存液中添加不同水平海藻糖、蔗糖和乳糖对猪精液冷冻保护的效果。结果表明,添加3种双糖均能提高精液的冷冻保护效果,其最佳添加水平均为0.035 g/mL,在该添加水平下,海藻糖组的精子活率、活力和精子质膜完整率分别为(46.34±0.52)%,(41.38±0.39)%和(43.51±1.78)%,与蔗糖组差异均不显著(P>0.05),但均显著高于乳糖和对照组(P<0.05);海藻糖组的有线粒体活性精子百分率和精子顶体完整率分别为(44.56±1.16)%,(64.09±0.81)%,显著高于蔗糖、乳糖和对照组(P<0.05),3种双糖均随添加水平升高精子的顶体完整率逐渐升高;海藻糖组获能处理前后的精子获能率分别为(3.68±0.07)%和(41.82±0.56)%,显著优于蔗糖、乳糖和对照组(P<0.05)。说明海藻糖对猪精液冷冻保护的效果最好,最佳添加水平为0.035 g/mL。 |
| 关键词: 猪精液 精液冷冻 海藻糖 蔗糖 乳糖 |
| DOI： |
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| 基金项目:陕西省科技攻关项目(2004K02-G11-03，2005K02-G03-03)；西北农林科技大学科研专项(05ZR100) |
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| Effect of trehalose,sucrose and lactoseon on cryopreservation of boar sperm |
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| Abstract: |
| In this study,the effects of adding three disaccharides (trehalose,sucrose and lactose) in the cryopreservative solution were compared.The results were summarized as follows:The effect of boar semen cryopreservation was markedly improved with three disaccharides,and their optimum level was 0.035 g/mL;At the level of 0.035 g/mL,the viability,motility,the percentages of mitochondrial activity and membrane integrity of frozen-thawed sperm with trehalose were (46.34±0.52)%,（41.38±0.39）%,(44.56±1.16)% and (43.51±1.78)% respectively,and there was no significant difference between trehalose and sucrose (P＞0.05),and both of them were higher than that of lactose group and the control（P＜0.05)；The percentage of intact acrosome of trehalose group was (64.09±0.81)%,and higher than that of the sucrose,lactose group,and the control (P＜0.05),and increased with the increasing level of three disaccharides;The percentages of capacitating boar sperm before and after capacitation treatment were （3.68±0.07）% and（41.82±0.56）%,and was better than that of lactose group and the control（P＜0.05）.To sum up,the effect of improving the frozen thawed sperm quality with trehalose was superior to sucrose,lactose and TCG control，and the optimum level was 0.035 g/mL. |
| Key words: boar semen cryopreservation trehalose sucrose lactose |
