| 摘要: |
| 采用不同剂量的60Coγ射线对辅酶Q10产生菌——三孢布拉氏霉JSF4菌株的孢子进行处理。结果表明，诱变剂量1.5 k Gy对孢子的致死率为85％，具有较好的诱变效果。经复筛，分离得到了1株F43菌株，其发酵平均单位达24.766 mg/L，较出发菌株提高了76％，且该菌株遗传性状稳定。 |
| 关键词: 60Coγ射线 辅酶Q10 三孢布拉氏霉 |
| DOI： |
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| 基金项目:江苏省“十五”科技攻关（工业）项目（BE2004031） |
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| Studies on the screening of mutant of Blakeslea trispora on CoQ10 induced by 60Coγ radiation |
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| Abstract: |
| The spore suspension of Blakeslea trispora was treated with different dosages of 60Co irradia tion.The study showed that when 1.5 kGy mutagenic dosage was provided,85 % of the spores died,resulting in a good mutagenic effect.By re-screening,a mutant F4-3 was obtained.Compared with the parent strain,its production of CoQ10 reached 24.766 mg/L,and the increase rate was 76%,and its genetic character was stable. |
| Key words: 60Coγ radiation coenzyme Q10 Blakeslea trispora |
