| 摘要: |
| 将12只装有永久性瘘管的成年雄性东北细毛羊随机分为4组，分别饲喂含0，100，200和300 mg/kg丝兰皂甙的饲粮，研究了不同水平丝兰皂甙对绵羊瘤胃内纤维素酶、总脱氢酶活性的影响，及其对全混合日粮有机物（OM）、干物质（DM）、中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）48 h瘤胃降解率的影响。结果表明，各试验组绵羊瘤胃内的总脱氢酶平均活性依次为95.62，97.36，99.48和101.54 U/mL，与对照组（第1组）相比，第4组总脱氢酶活性提高了6.2％；纤维素酶平均活性第2、3和4组分别较对照组（44.66 U/mL）提高了5.2％，8.5％和16.7％，其中第4组同1、2组相比达显著水平（P<0.05），第1、2、3组间差异不显著（P>0.05）;第2、3、4组OM瘤胃48 h降解率分别为59.90％，62.28％和64.65％，均高于对照组的58.74％，其中第4组高于对照组10.06％，二者差异显著（P<0.05）；DM和NDF48 h降解率均以第4组最高，分别为57.54％和34.12％，显著高于对照组的13.07％和13.06％；1～4组ADF 48 h降解率虽有上升趋势，但4组间差异不显著（P>0.05）。 |
| 关键词: 丝兰皂甙 绵羊 纤维素酶 总脱氢酶 瘤胃48h降解率 |
| DOI： |
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| 基金项目:黑龙江省青年科学基金(QC05C71)；中科院东北地理与农业生态研究所知识创新前沿项目（KZCX3-3W-NA-10） |
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| Effects of sarsa-saponine on enzyme activity and diet nutrient 48 h apparent digestibility on rumen |
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| Abstract: |
| Twelve male adult local sheep with permanent rumen cannula were used to study the effect of four levels of Sarsa-saponine(0,100,200 and 300 mg/kg) on Enzyme Activity and Diet Nutrient 48 h Apparent Digestibility on Rumen.The experiment was a completely randomized block design.Enzymes activity of ruminal liquid were measured 2 h after feeding every seven days during the twenty-eight days.According to the results of the five days,the changing trends of activities of total dehydrogenase and cellulase were increased as the level of Sarsa-saponine increased.Cellulase activity was distinctly influenced at a level of 300 mg/kg compared with control and 100 mg/kg group.The effect of the three levels of Sarsa-saponine on the rumen degradability of DM,OM,NDF and ADF in 48 h were evaluated with nylon bags.The results showed that the trends of degradability of nutrient material increased as Sarsa-saponine supplement increased.Rumen degradability of OM,DM,NDF in 48 h for 300 mg/kg Sarsa-saponine was distinctly influenced compared with control and 100 mg/kg group(P<0.05),but the rumen degradabilities of ADF in 48 h in four groups were not distinctly influenced. |
| Key words: sarsa-saponine sheep cellulase total dehydrogenase 48 h apparent digestibility on rumen |
