| 摘要: |
| 研究了不同溶氧（DO）浓度对微生物谷氨酰胺转胺酶（MTG）发酵的影响。结果表明．当溶氧浓度为2.88 mg/L时，MTG活性和菌体下重最高，分别达到4.32 U/mL和23.5 g/L，底物利用率为81.6％；当溶氧浓度继续升高时，发酵后期MTG活性下降显著。为此采用了分阶段溶氧控制策略，即在0～34h控制溶氧浓度为2.88 mg/L，34 h后控制溶氧浓度为0.96 mg/L，最终MTG活性达到4.72 U/mL，菌体干重达到27.32 g/L，较恒定2.88 mg/L溶氧浓度时分别提高了9.26％和16.26％．其底物利用率达83.2％。 |
| 关键词: 谷氨酰胺转胺酶 溶氧浓度 分段控制策略 酶活性 底物利用率 |
| DOI： |
| 分类号:TS201.3 |
| 基金项目: |
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| Effects of DO concentrations on Microbial Transglutaminase Fermentation |
|
JI Guo-Dong ZHOU Bo GAO Hong-Liang CHANG Zhong-Yi YANG Jun
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| Abstract: |
| The effects of DO concentrations on MTG batch fermentation were studied.The results showed,when DO concentration was 2.88 mg/L,MTG activity and dry cell weight reached the highest,which were 4.32 U/mL and 23.5 g/L respectively,and substrate utilization ratio was 81.6 %.However,the higher DO concentration could remarkably reduce the MTG activity at later phase.Therefore,the strategy of stepwise DO concentration control MTG batch fermentation was introduced:at 0～34 h,DO concentration were controlled at 2.88 mg/L,and after 34 h,it went down from 2.88 mg/L to 0.96 mg/L.And finally,MTG activity reached 4.72 U/mL and dry cell weight reached 27.32 g/L,9.26 % and 16.26% higher than when DO conantration was 2.88 mg/L respectively,and substrate utilization ratio was 83.2%. |
| Key words: Microbial Transglutaminase DO concentration the strategy of stepwise control enzymeactivity substrate utilization |
