| 摘要: |
| 采用国内不同厂家生产的促卵泡刺激素（FSH）和加拿大进口的FSH，进行了无角道塞特母羊超排处理，对不同给药方式、首次超排与重复超排、经产羊与青年羊、春季超排与秋季超排、自然发情与诱导发情及未知发情周期的超排效果进行了对比研究，同时研究了母羊卵巢黄体退化、有无卵泡及推迟发情对超排效果的影响。结果表明，加拿大进口FSH的超排效果最好，国产FSH以宁波＋中科院激素组合的超排效果较好；重复超排对超排效果无显著影响；经产羊的超排效果优于青年羊；自然发情母羊的超排效果优于诱导发情母羊和未知发情周期母羊；黄体退化母羊州收可用胚的数量明显较低；卵巢上有无卵泡对超排效果无影响；推迟发情母羊的超排效果明显低于正常发情母羊。 |
| 关键词: 无角道塞特绵羊 促卵泡刺激素 超数排卵 胚胎移植 |
| DOI： |
| 分类号: |
| 基金项目:农业部“948”项目“引进波利帕羊改良繁育我国肉用绵羊”（96207） |
|
| Study of technologies of superovulation on Poll Dorset sheep |
|
|
| Abstract: |
| This study was conducted to investigate the superovulation(SOV) efficiency of two different FSHs (made in Canada and China respectively ) on Poll Dorset sheep. Therefore,the experiment compared several factors on SOV,including ways of drug administration,repeated SOV,multi- or primiparous ewes, season,natural estrous and induced estrous and unknown estrous cycles. At the same time,the effects of corpora lutea degradation, follicles and estrous delayed on SOV were observed. The results of this study show that FSH (CA) had the best effect on SOV,the second was the combination of FSH (made in Ningbo and in Chinese Academy of Science). Repeated superovulation had no significant effect on SOV efficiency. Multiparous ewes had better SOV result than primiparous ewes. SOV was better in natural estrous ewes than induced estrous and unknown estrous cycle ewes. Useable embryo recovery was significantly low in ewes of corpora lutea degradation. Follicles' existing in ovary or not had no significant difference on SOV and the efficiency of SOV was significantly lower in the estrous delayed ewes than in normal estrous ewes. |
| Key words: poll dorset sheep FSH superovulation embryo transfer |
