| 摘要: |
| 以马哈利樱桃砧木的嫩梢茎尖为外植体进行离体培养,筛选出的起始培养基为MS+6-BA 0.2～0.5 mg/L；最佳增殖培养基为MS+6-BA 2.0 mg/L+IAA 0.5 mg/L+GA3 0.5 mg/L，此时增殖倍数达7.06，平均苗高2.79 cm；最佳生根培养基为1/2 MS+IAA 2.0 mg/L或1/2 MS+IBA 0.6 mg/L，暗培养7 d后移至温室或大棚,在自然光下生根炼苗，生根率达85%以上，单株平均根数3～6条，根系白嫩，组培苗生长整齐健壮；以营养土为基质,用50 mg/L生根粉泥浆溶液处理生根组培苗的移栽成活率达87.0%以上。该技术体系适宜工厂化生产的要求。 |
| 关键词: 马哈利樱桃 组织培养 快繁体系 |
| DOI： |
| 分类号: |
| 基金项目:国家“十五”攻关项目（2001BA701A35）；教育部重点项目（660514） |
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| Study on rapid propagation technology of Prunus mahaleb |
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| Abstract: |
| Vegetative axillary shoots were excised from field trees of Prunus mahaleb,then surfacesterilized and cultured on MS medium with 0.2-0.5 mg/L 6-BA. The results showed that optimal medium for shoot proliferation were MS medium with 2.0 mg/L 6-BA and 0.5 mg/L IAA and 0.5 mg/L GA3. On this medium, the average propagation multiple was 7.06 and average height of plantlets was 2.79 cm. Roots were induced on MS medium containing half-strength macroelements and 2.0 mg/L IAA or 0.6 mg/L IBA. After being cultured 7 days in the dark,axenic plantlets were transplanted to greenhouse for rooting and acclimation in the normal light ,more than 85% plantlets were induced strong roots,the average number of roots each strain were 3-6. The plantlets dealt in slurry with 50 mg/L ABT1 were transplanted into nutrition soil and survival rate was more than 87.0%. This propagation way can satisfy the need of factory production. |
| Key words: Prunus mahaleb tissue culture rapid propagation system |
