| 摘要: |
| 将克隆并测序的鸡卵清蛋白基因5′端调控序列和鸡IL-2基因cDNA,以串联方式置于真核表达载体pcDNA3的CMV启动子下游，构建了鸡输卵管定位表达载体pcDNA3-OVP-IL2将真核表达载体pcDNA3-IL2和构建的输卵管定位表达载体pcDNA3-OVP-IL2分别转染鸡输卵管上皮细胞，激素诱导72 h后，用淋巴细胞转化试验检测细胞培养液中IL-2的表达水平及生物学活性。结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3-OVP-IL2的输卵管上皮细胞表达产物在1∶256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。转染pcDNA3-IL2载体的输卵管上皮细胞虽有IL-2表达，但水平较低。 |
| 关键词: 鸡 IL-2基因cDNA 输卵管定位表达 转染 |
| DOI： |
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| 基金项目:河南省自然科学基金项目（0311031000） |
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| Construction of chicken IL-2 cDNA oviduct-specific expression vector and its expression in oviduct-cells |
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| Abstract: |
| The oviduct-specific expression plasmid pcDNA3-OVP-IL2，containing chicken IL-2 gene cDNA, was constructed by linking up CMV and chicken ovaibumin gene 5′-flanking regulatory region.The three plasmids,pcDNA3,pcDNA3-IL2 and pcDNA3-OVP-IL2,were mixed with Liposome and transfected chicken oviduct cells.After 72 h induced by Progesterone and Glucocorticoid as well as Insuline,biological activities of IL-2 were assayed with morphological docimasia method.The results showed that the plasmids of pcDNA3-OVP-IL2 and pcDNA3-IL2 could express IL-2,and the IL-2 could augment T cell proliferation.The expression level of pcDNA3-OVP-IL2 was higher than that of pcDNA3-IL2 so that the product could augment T cell prolifieration after 1∶256 dilution. |
| Key words: chicken IL-2 gene cDNA oviduct-specific express transfection |
