| 摘要: |
| 报道了1株粉纹夜蛾转P35基因工程细胞系，命名为Tn5B-35。苜蓿丫纹夜蛾核型多角体病毒(Autographa californica nuclear polyhedrosis virus，AcMNPV)在该细胞系中连续传代至25代，与原始细胞系Tn5B1-4相比较，其感染率、多角体产量、滴度以及杀虫毒力的变化趋势均比较平稳，并未出现“传代效应”。 |
| 关键词: 核型多角体病毒 昆虫细胞系 粉纹夜蛾 传代效应 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(30370963) |
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| Serial passage of AcMNPV-1A in a transfected P35 gene cell line |
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| Abstract: |
| A new transfected P35 gene cell line,Tn5B-35,was derived by Trichoplusia ni Tn5B1-4 cell line.Autographa californica nuclear polyhedrosis virus (AcMNPV) was passaged in Tn5B-35 cells for 25 passages continuously and there was no significant change in morphology of the susceptibility,the changing trends of the occlusion bodies（OBs）yield,virus titer,and the larval corrected mortality after serial passage of the virus were stable,and there was no passage effect. |
| Key words: nuclear polyhedrosis virus insect cell lines Trichoplusia ni passage effect |
