| 摘要: |
| 对1株拮抗链霉菌No.24菌株的发酵条件进行了初步研究，确定了其最佳培养基组成及培养条件。单因素发酵试验和正交试验结果表明，碳源以30 g/L葡萄糖和30 g/L小米最佳，有机氮源以10 g/L黄豆饼粉最好，无机氮源以2 g/L (NH4)2SO4最好，最适发酵时间为96 h，最适发酵培养温度为32 ℃，最适初始pH值为7.2～7.4，最佳振荡频率为210 r/min，装液量为40 mL/250 mL。 |
| 关键词: No.24菌株 发酵条件 链霉菌 |
| DOI： |
| 分类号: |
| 基金项目:国家863高技术计划项目(2002AA245121) |
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| Studies on the optimization of fermentation conditions of antagonistic streptomyces No.24 |
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| Abstract: |
| The fermentation conditions of 1 No.24 strain of antagonistic strepomyces were studied.The composition of fermentation medium and cultural conditions were confirmed.The single factor and orthogonal experiment indicated that the optimal carbon source was 30 g/L glucose,and millet 30 g/L；the optimal nitrogen source was 10 g/L Soybean cake powder and 2 g/L (NH4)2SO4；The optimal fermentation time was 96 h;The optimal cultivation temperature was 32 ℃ with optimal pH 7.2-7.4.The most suitable shake was 210 r/min,and the volume of cultivated liquid in 250 mL conic bottle was 40 mL. |
| Key words: No.24 strain fermentation condition strepomyces |
