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苦皮藤素Ⅳ对东方粘虫肌细胞超微结构的影响
廉喜红1, 刘惠霞1, 吴文君1
西北农林科技大学 植物保护学院
摘要:
采用电子显微镜技术,研究了苦皮藤素Ⅳ对东方粘虫(Mythimna separata Walker)肌肉系统的影响。电镜观察发现,苦皮藤素Ⅳ对东方粘虫成虫飞行肌和幼虫体壁肌均具有毒性;中毒试虫肌细胞,特别是质膜和内膜系统以及肌原纤维发生明显病变,表现为肌膜破坏、脱落,线粒体肿胀、崩解,内质网扩张,肌丝排列紊乱乃至消解;麻痹期间细胞内产生晶状包含体。结果表明,肌细胞可能是苦皮藤素Ⅳ的又一作用部位。
关键词:  苦皮藤素Ⅳ  东方粘虫  肌细胞
DOI:
分类号:
基金项目:国家自然科学基金重点项目(30130130)
Ultrastructural effect of Celangulin Ⅳ on the myocyte of Mythinma separata (Walker)
Abstract:
Studies have been conducted with electric microscope on the ultrastructure of myocyte on the armyworm,Mythinma separata.The main results are as the followings:Celangulin Ⅳ had myotoxity on both the flight muscles of the adult and the body muscles of the larvae.The myocyte,especially the muscular membrane and internal membrane system and myofibrils went progressive myotoxity.Mitochondria were swollen and broke down.Sarcoplasmic reticulum was expanded.Myofibril arrangement was out of order and even dissolved.The cryinstalline inclusions appeared on the paralysis stage.The results indicate that the myocyte is possibly another target of Celangulin Ⅳ.
Key words:  Celangulin Ⅳ  Mythinma separata  myocyte