| 摘要: |
| 通过有效的细胞培养方法获得了牛乳腺上皮细胞系，并系统观察了乳腺上皮细胞的长出、贴壁、聚集、迁移、分裂、分化、凋亡等一系列形态变化。结果发现，原代培养的乳腺上皮细胞大多数呈卵圆形，细胞之间连接成片，单层生长，如鹅卵石铺过路面，乳腺上皮细胞和成纤维细胞混生时分区生长，界线明显。传代的乳腺上皮细胞呈岛屿状聚集生长，细胞核呈圆形或椭圆形，核仁2～4枚；在含雌激素的培养液中，细胞出现双核或多核现象，但仍表现一定的接触抑制现象。多次传代后的乳腺上皮细胞含不同的细胞类型，通过光镜观察，部分细胞仍保持较快的分裂增殖能力；部分细胞渐渐分化，出现长形细胞、三角形细胞。上皮细胞增殖分化可形成圆顶型结构，乳腺上皮细胞可产生并分泌乳汁，分泌到细胞外的乳汁流动形成网状结构。 |
| 关键词: 体外培养 牛 乳腺上皮细胞 细胞形态 |
| DOI： |
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| 基金项目:国家“863”高技术研究发展计划项目(2001AA213081) |
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| Study on the appearance of vitro-cultured bovine mammary epithelial cells |
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| Abstract: |
| Bovine mammary epithelial cell lines were obtained by culturing tissue fragment.Then the appearance of these cells were studied by light microscope.The results are as follows:The fibroblast cell and the bovine mammary epithelial cells grow in different areas.The bovine mammary epithelial cells were cultured in vitro through single-cell afloat,which formed island monolayer aggregates,then the cells propagated and formed dome-like construction.The cell nucleus is circular or oval with 2-4 cores.In the culture medium with estrogen,the nucleus type changed.The mammary epithelial cells can be developed into many cell types,the majority of which are short shuttle-like or guboidal.The cells connected with each other properly and formed net-like construction. |
| Key words: in vitro culture mammary epithelial cell the appearance of cells |
