| 摘要: |
| 将新城疫病毒(NDV)F48E9株融合蛋白(F)基因1 700 bp克隆到真核表达载体pIRES中，构建成表达F基因的载体pIRESF，然后将包含F基因及其上游的内含子和下游的polyA的2 900 bp DNA片段再克隆入包含gB启动子的SK载体中，并使其克隆到gB启动子600 bp的下游，最后将包含gB 启动子和F基因表达盒3 500 bp克隆到包含MDV CVI988非必须片段US10 的载体SK中。该研究为进一步在细胞中转染并获得表达F基因的MDV CVI988重组病毒奠定了基础。 |
| 关键词: 新城疫病毒 F基因 马立克氏病病毒CVI988转移质粒载体 |
| DOI： |
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| Construction of recombinant Marek's disease virus CVI 988 strain transferring plasmid vector expressing F gene of NDV F48E9 strain |
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| Abstract: |
| The NDV F48E9 strain fusion protein gene was cloned into the multi clone site of eukaryotic expression vector pIRES to form PIRESF.Then 2 900 bp DNA fragment of F gene with IVS and polyA was cloned into multi clone site of vector SK with gB promotor of MDV.Finally,3 500 bp DNA fragment of F gene with gB promotor of MDV was cloned into vector SK containing US10,the nonessential fragment of MDV CVI 988.The research lays a foundation of obtaining recombinant MDV CVI 988 expressing NDV F gene. |
| Key words: NDV F gene:MDV CVI 988 transferring plasmid vector |
