| 摘要: |
| 取10头隐性乳房炎患牛乳汁，采用北京亚临床乳房炎快速诊断液(BMT试剂)，对泌乳黑白花奶牛的乳汁进行了病原菌分离鉴定，结果共分离出金黄色葡萄球菌、乳房链球菌和大肠杆菌3种病原菌。将蒲公英、王不留行等中药水煎、去渣、浓缩制成相当于2 g/mL的生药液，然后稀释为1.5，1.0，0.5 g/mL 3种质量浓度，用优质定性滤纸制成相应浓度的药敏片进行中药体外抑菌试验，结果表明，该中药对以上3种病原菌均有明显的抑制作用。 |
| 关键词: 中药复方 奶牛 隐性乳房炎 体外抑菌试验 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(39870582) |
|
| nhibition to pathogens of cow recessive mastitisby Chinese herb compound prescription in vitro |
|
|
| Abstract: |
| The pathogens of milk from ten positive cows was separated and indentifified,the fast diagnostic reagent of the sub-clinical mastitis from Bejing (BMT) was used to examine the white-black cow from The Cow Breeding Center in Xi'an and The Second Team in Cao-Tan Farm.The results indicated that there were three kinds of pathogens in the milk:Staphylococcus aureus,Streptococcus uberis and Escherichia coli.Chinese herbs such as taraxacum,vaccaria segetalis and so on were made into medicative liquid,of which the concentration was 2 g/mL by decocting in water,eliminating lees and concentrating.After that,it was diluted into 1.5 g/mL,1.0 g/mL and 0.5 g/mL respectively.Then,they were made into sensitive tablets using high quality filter papers correspondingly.Those tablets were used to test their inhibition against the isolated pathogens.The results showed that this compound prescription could inhibit the three pathogens obviously. |
| Key words: Chinese herbs compound prescription cow recessive mastitis inhibition against pathogens in vitro |
