| 摘要: |
| 为了完善非洲菊组培快繁体系和推动非洲菊种前工厂化生产，本试验以非洲菊叶片为外植体，对影响非洲菊愈伤组织诱导的基本培养基、激素种类及其浓度等关键因素进行了研究。结果表明,基本培养基以MS诱导愈伤组织效果最好,诱导率可达78.3%；2,4-D比NAA和IBA能够较快地诱导叶片产生愈伤组织，其中以1.0 mg/L 2,4-D诱导率最高，可达100%,且形成愈伤组织多；6-BA诱导愈伤组织的效果优于KT和TDZ,2.0 mg/L 6-BA的诱导率为89.7%；叶片6-BA 2.5 mg/L+2,4-D 0.5 mg/L+NAA 0.4 mg/L培养基上愈伤组织诱导率最高，诱导出的愈伤组织生长快，并可分化出不定芽。 |
| 关键词: 非洲菊 叶片 培养基 植物激素 组织培养 |
| DOI： |
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| 基金项目:陕西省“十五”科技攻关项目(99K04-G7) |
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| Effects of media and plant hormones on callus induction of gerbera leaves in vitro |
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| Abstract: |
| The effects of the basic media,the types and concentrations of plant hormones on callus induction of gerbera leaves were studied in order to develop the gerbera tissue culture system and drive its young plants industrialization.The results showed that MS medium was the best on callus induction,induction rate was 78.3%;2,4-D was faster on callus induction than NAA and IBA,thereinto,the medium of 1.0 mg/L 2,4-D was the highest,its induction rate was 100%,and leaves formed more callus.6-BA had better effect than KT and TDZ,the induction effect of 2.0 mg/L 6-BA was 89.7%.Leaves had the highest induction rate of callus on the medium of MS+6-BA 2.5 mg/L+2,4-D 0.5 mg/L+NAA 0.4 mg/L.These calli grew fast.Adventitious buds could be obtained from these calli quite well. |
| Key words: gerbera leaf medium plant hormone tissue culture |
