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外源甜菜碱对盐胁迫枸杞生长及膜脂过氧化的影响
惠红霞1, 许 兴1, 李前荣2
1.宁夏农业生物技术重点实验室;2.宁夏农学院
摘要:
对枸杞扦插苗分别进行清水处理(CK)、盐胁迫(6 g/kg NaCl)处理和盐胁迫 外源甜菜碱(6 g/kg NaCl+1 mmol/L甜菜碱)处理,分别在处理后第4,8,12和16天测定其生理变化,研究外源甜菜碱对盐胁迫枸杞生长及膜脂过氧化的影响。结果表明,外源甜菜碱能显著提高枸杞叶片相对含水量和株高净增长量,能明显促进枸杞生长;甜菜碱能使盐胁迫枸杞的丙二醛含量降低,使超氧化物歧化酶、过氧化物酶和过氧化氢酶活性升高,β-胡萝卜素含量明显增加。说明外源甜菜碱有利于保护细胞膜结构,降低盐胁迫对枸杞的伤害。
关键词:  甜菜碱  盐胁迫  枸杞  膜脂过氧化
DOI:
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基金项目:国家重点基础研究发展规划项目(G1999011700)
Effect of exogenous betaine on the growth and membrane lipid peroxidation in Lycium barbarum under salt stress
Abstract:
Using water (CK) and solution of NaCl (6 g/kg) to treat Lycium barbarum seedling as control,we add betaine to solution of NaCl (6 g/kg) to treat Lycium barbarum seedling for spans of 4,8,12 and 16 days,and determine the physiological changes in leaves of Lycium barbarum seedling.Effect of exogenous betaine on the growth and membrane lipid peroxidation in Lycium barbarum under salt stress was studied.The results indicated that exogenous betaine could increase the relative water content and height of plants,improve the growth,decrease the content of MDA,increase the activities of SOD,POD and CAT and the content of β-carotens of Lycium barbarum seedling under salt stress.The results suggested that exgenous betaine would be useful to protect cell membrane structure,therefore the growth inhibition caused by salt stress were mitigated by exgenous betaine in Lycium barbarum.
Key words:  betaine  salt stress  Lycium barbarum  membrane lipid peroxide