| 摘要: |
| 利用PCR-RFLP(HinfⅠ、HaeⅢ和MspⅠ 3种限制性内切酶)分子标记技术，检测了杜洛克猪、长白猪、大白猪、内江猪、荣昌猪、汉江黑猪、汉白猪、八眉猪和野猪等8个猪种，共计265头猪心脏脂肪酸结合蛋白基因5′-上游区和第二内含子区的遗传变异。结果表明，在HinfⅠ-RFLP位点上，上述猪种和野猪均存在多态性，等位基因H的频率分别为0.750 0，0.718 8，0.916 7，0.333 3，0.125 0，0.690 9，0.116 7，0.850 0和0.937 5；除汉江黑猪（P<0.05）和野猪（P<0.01）外，其余猪种的基因频率和基因型频率都处于Hardy-Weinberg平衡状态（P>0.05）；大白猪、八眉猪、汉江黑猪、汉白猪和野猪表现为低度多态性（PIC<0.25），杜洛克猪、长白猪、内江猪和荣昌猪为中度多态性（0.25<PIC<0.5）；而在HaeⅢ-RFLP和MspⅠ-RFLP位点上，仅内江猪、荣昌猪、汉江黑猪和八眉猪为单态。 |
| 关键词: 猪 H-FABP基因 PCR-RFLP 分子标记 遗传变异 |
| DOI： |
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| 基金项目:国家自然科学基金项目(200168572) |
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| PCR-RFLP molecular marker of pig H-FABP gene |
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| Abstract: |
| In this experiment,genetic variations of 5′-upstream region and the second intron in 265 pig H-FABP gene including Duroc,Landrance,Largewhite,Neijiang pig,Rongchang pig,Hanjiang Black pig,Hanzhong White pig,Bamei pig and wild pig were checked by PCR-RFLP Molecular Marker with HinfⅠ,HaeⅢ and MspⅠ.The result showed that for HinfⅠ-RFLP,the above pig breeds had polymorphism and frequencies of allele H are 0.750 0,0.718 8,0.916 7,0.333 3,0.125 0,0.690 9,0.116 7,0.850 0 and 0.937 5 respectively;expect for Hanjiang Black pig(P<0.05) and wild pig (P<0.01),frequencies of gene and genotype in other breeds are in equilibrium of Hardy Weinberg (P>0.05);largewhite,Bamei pig,Hanjiang Black pig,Hanzhong White pig and wild pig present low polymorphism (PIC<0.25) while other breeds have mediat polymorphism（0.25<PIC<0.5）.Among tested breeds,only 4 Chinese local pig breeds have no polymorphism at the HaeⅢ-RFLP and MspⅠ-RFLP sites. |
| Key words: pig H-FABP gene PCR-RFLP molecular marker genetic variation |
