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植物甜菜碱醛脱氢酶基因研究进展
刘振林1, 戴思兰1
北京林业大学 园林学院
摘要:
对近年来植物甜菜碱醛脱氢酶(BADH)基因的研究现状进行了系统分析。对目前已分离到的10个不同种植物BADH基因的序列分析发现:该基因编码区序列趋于保守;在不同物种间存在拷贝数不同的差异;其表达也存在转录或转录后水平的差异,具有组织特异性、组成型和诱导型的不同表达类型;氨基酸序列同源性反映的种间关系与这些种的系统分类位置一致,表明该基因的进化与植物的系统进化间具有密切关系。此外,还对信号肽区域、外显子剪切位点以及BADH定位的变化等进行了分析。
关键词:  甜菜碱醛脱氢酶  基因结构  表达特性  系统进化
DOI:
分类号:
基金项目:北京市自然科学基金项目(5022008)
Studies on betaine aldehyde dehydrogenase (BADH) gene in plants
Abstract:
A comprehensive study on the reports of plant betaine aldehyde dehydrogenase (BADH) gene in recent years has been made in the paper.The analysis based on 10 different BADH genes indicates that the sequences of coding regions tend to be conservative.The locations of splicing sites between exons are consistent,introns and flanking regions have no obvious homology.The expression of BADH genes is different on DNA level,the copies of the genes are different quantitatively,and it is different on transcription of post-transcription level,while tissue-specific,constitutive and stress-induced expression type are all existed.The change of the location of BADH and the agreement of different species' relationship which is shown by the homology of amino acid sequence with these plants' phylogenetic relationship indicate that the development of BADH gene is highly relevant to the phylogenetic relationship of plants.
Key words:  betaine aldehyde dehydrogenase  gene structure  expressing characterization  phylogeny