| 摘要: |
| 从伤诱导的黄河蜜甜瓜(Cucumis melo ev.Huanghemi)成熟果实的中果皮组织中分离总RNA，经反转录和PCR扩增得到974bp的cDNA片段，克隆到质粒载体pGEM-3zf，经测序与Genbank中甜瓜1-氨基环丙烷1-羧酸合成酶(1-amino-cyclopropane-1-carboxylie acid synthase，ACS)基因同源性为99％。克隆片段经双酶切消化，反向插入到植物表达载体pBI121的35S启动子和NOS终止子之间，构建成ACS基因的反义表达载体。 |
| 关键词: 黄河蜜甜瓜 ACS基因 反义载体 |
| DOI： |
| 分类号: |
| 基金项目:澳大利亚国际农业中心项目(PHT/1998/140) |
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| Molecular cloning of a muskmelon cDNA fragment encoding an amino-cyclopropane-1-carboxylie acid (ACC) synthase and construction of antisense expression vector |
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| Abstract: |
| A cDNA fragment of an amino-cyclopropane-1-carboxylie acid (ACC) synthase was amplified by Reverse Transcription Polymerase Chain Reaction (RT-PCR) from wounded mesocarp tissue of mature muskmelon (Cucumis melo cv.Huanghemi) and cloned into a vector pGEM-3zf.DNA sequencing showed that the cloned fragment shared highly homogeneity to the cDNAs of ACC synthases from other Cucubitae plants.The cloned cDNA of ACC synthase was further introduced into a binary vector pBI121 in a reverse orientation with its upstream promotor (CaMV 35S),giving an expressing plasmid containing the antisense ACC synthase gene. |
| Key words: muskmelon ACC synthase gene antisense expression vector |
