| 摘要: |
| 用电子显微镜技术和细胞化学技术，研究了羟菌唑对油菜菌核病菌(Sclerotinia sclerotiorum)生长发育的影响。扫描电镜观察发现，药剂处理后病菌菌丝生长明显受到抑制，菌丝粗细不均匀，呈不规则肿大或缢缩，分枝明显增多。超微结构研究表明，药剂处理后，病菌菌丝细胞壁不规则加厚，以菌丝顶端细胞壁加厚尤为明显，并有大量电子致密度高的物质存在；与对照菌丝相比，病菌菌丝隔膜肿大、畸形，发育受阻；有的菌丝体内有新菌丝产生，且染色较深。细胞化学标记分析结果表明，药剂处理后，病菌菌丝细胞壁的β-1,3-葡聚糖和几丁质的标记密度明显高于对照菌丝，说明药剂处理后，这2种成分在细胞壁内显著增加。病菌菌丝细胞壁成分的变化表明，羟菌唑影响了病菌细胞结构及其功能。 |
| 关键词: 油菜菌核病菌 羟菌唑 超微结构 β-1,3-葡聚糖 几丁质 |
| DOI： |
| 分类号: |
| 基金项目:国家杰出青年基金项目(30125031)；陕西省科技攻关项目(2001K02-G4) |
|
| Effects of fungicide metconazole on the hyphal development of Sclerotinia sclerotiorum on canola |
|
|
| Abstract: |
| The effects of metconazole,Abstract: systemis fungicide from the triazol group were examined on morphology,structure, cell wall components of Sclerotinia sclerotiorum by means of electron microscopy and immunocytochemical labelling techniques.The studies rewealed that metconazole not only inhibited the hyphal growth,but also caused a series of marked morphological and structural alterations of hyphae.These changes included irregular swelling,excessive branching and many tumors on the surface of hyphae,considerable thickening of the hyphal cell walls,particularly at the hyphal tip region,The septum development of the hyphae was inhibited and became malformed.Using cytochemical techniques,the labelling densities of chitin and β-1,3-glucan in the cell wall of the fungicide-treated hyphae were increased dramatically comparing with those of the untreated control hyphae,indicating that the detrimental effects of the fungicide on structure and function of the hyphal membranes resulted in the changes of the cell walls compositions. |
| Key words: Sclerotinia sclerotiorum metconazole ultrastructure β-1,3-glucan chitin |
