| 摘要: |
| 用对比试验和正交试验对甲鱼酶解液化技术进行了研究。结果表明，木瓜蛋白酶的较佳酶解条件为酶用量（E/S）6 g/kg，酶解温度65 ℃，pH值5.5，在此条件下酶解8 h后氨基态氮含量为0.262 g/kg，木瓜蛋白酶酶解速度慢;胃蛋白酶的较佳酶解条件为酶用量（E/S）42 g/kg，酶解温度60 ℃，pH值1.5，在此条件下酶解6 h后氨基态氮含量为0.286 g/kg;再将这2种酶在同一底物下酶解,胃蛋白酶酶解速度较木瓜蛋白酶快，6 h后酶解液氨基态氮含量为0.179 3 g/kg，而木瓜蛋白酶8 h后酶解液氨基态氮含量为0.159 0 g/kg;胃蛋白酶酶解甲鱼蛋白液的颜色较木瓜蛋白酶的好;用2种酶复合酶解时，酶解效果较单独使用胃蛋白酶的效果差;酶解液经硅藻土过滤后，酶解液色泽呈乳清色，澄清透亮,适用于制作各种甲鱼保健食品。 |
| 关键词: 甲鱼 酶解 氨基态氮 |
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| The technology of enzymatic hydrolysis for the muscle of Trionys sinensis |
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| Abstract: |
| This paper is comparative research of enzymatic hydrolysis condition of subtilisin to Trionys sinensis protein.The analysed results of the orthogonal experiment indicate that the optmum conditions for the papain enzymatic hydrolysis were enzyme concentration 6 g/kg,temperature 65 ℃,pH 5.5,taking time 8 h.Then the content of amino nitrogen was 0.262 g/kg.Also,the analysed results of the orthogonal experiment indicate that the optmum conditions for the pepsin enzymatic hydrolysis were enzyme concentratio 42 g/kg,temperature 60 ℃,pH 1.5,taking time 6 h.Then,the content of amino nitrogen was 0.179 3 g/kg.Pepsin enzymatic hydrolysis with better result than papain enzymatic hydrolysis,with pepsin taking time 6 h.The content of amino nitrogen was 0.179 3 g/kg,but the content of amino nitrogen was 0.179 3 g/kg with papain taking 8 h.Enzymes were inactivated at 85 ℃ for 15 min,and the hydrolysate was filtered with diatomite.With bright colour and good taste,the hydrolysate was very good raw material for health food. |
| Key words: Trionys sinensis enzymatic hydrolysis amino nitrogen |
