| 摘要: |
| 对用发酵法从鲁氏毛霉中制备、分离、提取甲壳素和壳聚糖的方法和工艺条件进行了系统研究。结果表明：(1)鲁氏毛霉较佳培养基组成为果糖20 g/L，牛肉膏15 g/L，K2HPO4 0.75 g/L，MgSO4 1.80 g/L，N-乙酰葡萄糖胺1.54 g/L；其较优发酵条件为温度28 ℃，周期48 h，pH5.0，摇瓶装液量200 mL/L。(2)从菌丝体中提取甲壳素和壳聚糖的工艺条件：除蛋白质为氢氧化钠20 g/L，温度115 ℃，时间60 min；酸处理为盐酸30 g/L，温度80 ℃，时间180 min。(3)采用较优发酵提取条件后,甲壳素产量可达1.328 g/L，壳聚糖产量达0.672 g/L，壳聚糖占菌粉干重的7%。 |
| 关键词: 鲁氏毛霉 发酵法 甲壳素 壳聚糖 |
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| A study on the preparing of chitin and chitosan by fermentation with Mucor rouxianus |
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| Abstract: |
| It was systematically studied on the methods and technology of preparing,isolating chitin and chtosan by fermentation with Mucor rouxianus.As the experiment indicating:(1) The optimal composition of fermentation medium for Mucor rouxianus was:fructose 20 g/L,beef extract 15 g/L,K2HPO4 0.75 g/L,MgSO4 1.80 g/L,acetyl glucosamine 1.54 g/L;The optimal condition of fermentation was:temperature 28 ℃,time 48 h,pH 5.0,amount of medium per flask 200 mL/L.(2) The conditions of isolating chitin and chitosan from the mycelium were:① isolating protein:NaOH 20 g/L,temperature 115 ℃,time 1 h;② acid treatment:HCl 30 g/L,temperature 80 ℃,time 3 h.(3) Under the optimal conditions of isolation:yield of Mucor rouxianus was:chitin 1.328 g/L,chitosan 0.672 g/L,percent of chitosan in dried mycelium was 7%. |
| Key words: Mucor rouxianus fermentation chitin chitosan |
