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氮源与无机盐对高浓度酒精发酵的影响
吕 欣1, 段作营1, 毛忠贵1
江南大学 工业生物技术教育部重点实验室
摘要:
在完全合成培养基条件下,就尿素、硫酸铵2种氮源和硫酸镁、磷酸二氢钾、氯化钙3种无机盐对1株产高浓度酒精的酿酒酵母的间歇发酵影响进行了初步研究。结果表明,与尿素相比硫酸铵是较好的无机氮源,发酵终了酒精浓度可达119.9 g/L;并初步得到该株酵母发酵所需各无机盐的临界浓度分别为:硫酸镁5 g/L,磷酸二氢钾5 g/L(或<5 g/L),氯化钙5 g/L。
关键词:  酿酒酵母  无机盐  氮源  酒精发酵
DOI:
分类号:
基金项目:科技部科技型中小企业技术创新基金无偿资助项目(01C26213200325)
Study on the effects of nitrogen sources and inorganic salts on ethanol fermentation
Abstract:
The effects of nitrogen sources and inorganic salt on high ethanol fermentation in synthetic medium were studied in this paper.Ammonium sulfate was the better nitrogen source between urea and ammonium sulfate,the ultimate ethanol concentration was 119.9 g/L when we used ammonium sulfate as nitrogen source.Moreover,the effects of calcium chloride,magnesium sulfate and potassium biphosphate on high ethanol fermentation were analyzed,the critical concentrations of these inorganic salt the Saccharomyces cerevisiae needed were gained.
Key words:  Saccharomyces cerevisiae  inorganic salt  nitrogen source  ethanol fermentation