| 摘要: |
| 以“春秋”花椰菜为试验材料，研究了影响下胚轴再生的若干因子。结果表明，6 d苗龄的下胚轴在附加2～3 mg/L 6-BA和1.0 mg/L NAA的MS培养基上培养，芽分化率最高，达93.33%，平均每个外植体产芽5.71个；AgNO3对下胚轴的细胞生长有明显的促进作用；下胚轴的下端比上端产芽早，并且较易产生不定芽。 |
| 关键词: 花椰菜 下胚轴 离体培养 植株再生 |
| DOI： |
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| 基金项目:科技部杨凌生物育种中心基金项目（1999-18）；陕西省自然科学基金(2001SM11)；陕西省农业分子生物学重点实验室基金项目 |
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| High frequency of shoot regeneration from hypocotyls explants in Brassica oleracea var. botrytis |
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| Abstract: |
| The hypocotyls explants from “Chunqiu” cauliflower were cultured and some factors affecting shoot regeneration were also studied.The results showed the highest frequency of shoot regeneration of hypocotyls explants from 6-d-old in vitro grown seedling was 93.33% and No.of bud per explant with shoots was 5.71,using the MS media containing the hormone combinations of 6-BA 2-3 mg/L and NAA 0.5 mg/L.It was also found AgNO3 (6 mg/L) was advantageous to the shoot formation;the lower segments of hypocotyls produced shoot earlier and easier than the upper segments. |
| Key words: cauliflower hypocotyls in vitro plant regeneration |
