| 摘要: |
| 将348枚预孵24～28 h的种蛋分成8组，部分去壳后分别以不同方法封口，研究其对鸡胚存活率及出雏率的影响。结果表明：蛋壳膜+封口胶（封口后连续3 d注射抗生素）、蛋壳膜+封口胶、封口胶+药棉、蛋壳膜+石蜡、蛋壳膜+蛋壳粉+石蜡5种封口方法对鸡胚的发育在19日龄前差异不显著（P＞0.05）；前4 组的出雏率分别为9.6%,5.1%,4.4%,2.4%。蛋壳膜、蛋壳膜+蛋壳粉+蛋清、蛋壳膜+蛋壳粉3种封口方法对鸡胚发育在19日龄前较差，与前5组比较差异显著(P＜0.05)。8种方法比较，前5种封口方法对用显微注射法制备转基因鸡更具有应用潜力。 |
| 关键词: 鸡胚 孵化率；转基因鸡；体外培养；去壳 封口 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目 （30170678） |
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| Effect of partially deshelled culture of chick embryos with eight sealing methods |
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| Abstract: |
| A total of 348 fresh eggs of Suqin-96 layers were divided into 8 groups after having being hatched for 24-28 hours.Each egg was ripped a 0.5-1.0 cm hole and the windows of each of the 8 groups were sealed with different shields,and observed the effect on chicken embryos survival rate and hatchability.The results were as followings:the 5 treated groups/sealing materials were:① eggshell membrane+wrap film (injecting antibiotic for successive 3 days after sealed);② eggshell membrane+wrap film;③ wrap film+cotton;④ eggshell membrane+sealing wax;⑤ eggshell membrane+eggs shell powdery remnant+seaking wax.The survival rates of these 5 treated groups were not significantly different (P>0.05) before the 19th day.The hatchabilities of the first 4 treated groups were 9.6%,5.1%,4.4%,2.4% respectively.The left 3 groups sealing materials were:⑥ eggshell membrane;⑦ eggshell membrane+egg shell powdery remnant+egg white;⑧ eggshell membrane+eggshells powdery remnant.The developments of these 3 groups'embryos were not good before the 19th days,and significantly lower than the first 5 groups (P<0.05).Compared with the 8 treatments,the first 5 treatments could be choosed for production of transgenic chicken in practical applications. |
| Key words: chicken embryos birthrate transgenic chick in vitro partially-deshelled sealing |
